Application of Enzyme-Linked Immunosorbent Assay (ELISA) in Cytokine Research

Application of Enzyme-Linked Immunosorbent Assay (ELISA) in Cytokine Research

Cytokines (CK) are a class of small molecular proteins with a wide range of biological activities that are synthesized and secreted by various tissue cells (mainly immune cells) through stimulation. CK can be divided into interleukin, colony-stimulating factor, interferon, tumor necrosis factor, growth factor, etc. According to its main functions, CK can be divided into interleukin, colony-stimulating factor, interferon, tumor necrosis factor, growth factor, etc. Cytokine research has very important theoretical and practical significance. It helps to clarify the immune regulation mechanism at the molecular level and is helpful to the prevention, diagnosis and treatment of diseases. As a highly sensitive and specific detection method, ELISA is widely used in cytokine research. ELISA to detect the levels of cytokines and their receptors has become an important aspect of basic and clinical immunology research.

Common Targets in Cytokine Research

  • Amphiregulin
  • Betacellulin
  • Cerberus
  • Cardiotrophin 1
  • Dermokine
  • Epiregulin
  • Myostatin
  • Gastrokine 2
  • Interleukin 4
  • Interferon Alpha 4
  • Oncostatin M
  • Interleukin 1 Epsilon
  • Interleukin 1 Zeta
  • Fibroblast Growth Factor
  • Placenta Growth Factor

Advantages of ELISA in Cytokine Research

  • Helps to explore the physiological functions of cytokines
  • Contribute to the early diagnosis of related diseases
  • Helps to understand the structure and function of cytokine receptors

Common Targets Detected by ELISA in Cytokine Research


Cardiotrophin-1 (CT-1) is a cytokine of the interleukin-6 family. CT-1 can promote myocardial fibroblasts to proliferate and secrete collagen, and at the same time make myocardial cells hypertrophy. It plays an important role in ventricular remodeling caused by diseases such as ischemic heart disease, hypertension and heart failure. ELISA to detect the concentration of CT-1 can be used to screen the therapeutic target of heart failure, and the concentration can be used as a prognostic indicator.


Epiregulin (EPR) consists of 46 amino acid residues and is a member of the epidermal growth factor family. EPR is an epidermal growth factor receptor ligand, produced by keratinocytes and fibroblasts. It can be used as a ligand for epidermal growth factor receptors and most members of the ERBB family of tyrosine kinase receptors. EPR has a variety of biological functions, which can inhibit the growth of certain tumor cells and promote the growth of various cells such as fibroblasts.

Fibroblast Growth Factor

Fibroblast growth factor (FGF) is a polypeptide secreted by the pituitary gland and hypothalamus, and there are several isoforms. FGF is considered to be a foci formation promoting factor, but it also has a positive side from the perspective of repair. It can promote the generation of a large number of osteoblasts and inhibit osteoclasts; it can also strengthen the bone marrow hematopoietic function, promote the production of stem cells, and then generate a large number of red blood cells and white blood cells.

Creative Diagnostics has been committed to the application of ELISA in cytokine research. Relying on strong R&D capabilities and rich experience, we produce many ELISA kits products for cytokine research, and we provide high-quality customized ELISA kits services, believable ELISA testing services, professional ELISA development services related to cytokine according to your needs. If you want more information, please be at liberty to contact us.


  1. Martínez-Martínez, E.; et al. CT-1 (Cardiotrophin-1)-Gal-3 (Galectin-3) Axis in Cardiac Fibrosis and Inflammation. Hypertension. 2019, 73(3): 602-611.
  2. Roskoski, R.J. The ErbB/HER family of protein-tyrosine kinases and cancer. Pharmacol Res. 2014, 79: 34-74.
  3. Ornitz, D.M.; Marie, P.J. Fibroblast growth factor signaling in skeletal development and disease. Genes Dev. 2015, 29(14): 1463-1486. 
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