Heavy metal contamination refers to environmental pollution caused by heavy metals/metalloid arsenic or its compounds. Heavy metal contaminants mainly come from industrial pollution, traffic pollution, and domestic waste pollution. Heavy metal contaminants are difficult to degrade in the environment and accumulate in animals, plants, and humans, thus endangering the environment and human health. Certain heavy metals in organisms can be transformed into more toxic organic compounds by microorganisms, causing serious public hazards. For example, "Minamata disease" (mercury contamination) and "Itai-Itai Disease" (cadmium contamination) occurred in Japan. Some heavy metal contaminants even have carcinogenic and teratogenic effects. For example, lead can synthesize carcinogens with our organic and inorganic substances. Studies have shown that the absorption of lead in modern humans has increased by about 100 times compared with primitive humans.
Heavy metals can interact strongly with proteins and various enzymes in the human body, making them inactive, and may also be enriched in certain organs of the human body, causing human body poisoning. The toxicity of heavy metal contaminants is not only related to the amount ingested into the body but also closely related to its existence. For example, the toxicity of alkylmercury is significantly greater than that of inorganic salts of divalent mercury ions; among the compounds of arsenic, arsenic trioxide is the most toxic. Due to human activities, the content of heavy metals in the environment has increased, the quality of the environment has deteriorated, and it has directly endangered human health. Therefore, we should pay attention to monitoring the heavy metal content in the environment. Given the simplicity and efficiency of the ELISA method, it can also be used for high-throughput monitoring and analysis, so it was developed for the detection of heavy metal pollutants.
Indirect Competitive ELISA, Sandwich ELISA
The process is according to the type of sample. The tissue sample needs to be crushed and homogenized first, and then vortexed and centrifuged. After evaporation at 45°C under nitrogen, a certain amount of supernatant was taken for measurement. The samples are generally stored at 4 or -20℃.
We have a professional R&D team, mature technical route, perfect quality management system, and provide high-quality after-sales service. In the heavy metal contaminants ELISA test, we can do the following for you.
Lead is a toxic metal that can accumulate in human and animal tissues. It enters the body through the skin, digestive tract, and respiratory tract. The main toxic effects are anemia, neurological dysfunction, and kidney damage.
Mercury and its compounds are highly toxic substances and can accumulate in the human body. Metal mercury in the blood enters the brain tissue and accumulates, and when it reaches a certain amount, it will cause damage to the brain tissue.
Cadmium is not an essential element of the human body. It is highly toxic and can accumulate in the human body, causing changes in the function of the urinary system. In addition, cadmium can interfere with calcium in bones. Long-term intake of trace amounts of cadmium can cause bone pain.
Chromium can accumulate in the human body. Ingestion of chromium pollutants can cause abdominal discomfort and diarrhea and other poisoning symptoms, cause allergic dermatitis or eczema, breathe in, irritate and corrode the respiratory tract, cause pharyngitis, bronchitis, etc.
Arsenic and its compounds are common environmental pollutants. Generally, it can enter the human body through water, air and food, causing harm. Arsenic compounds are all toxic, and trivalent arsenic compounds are the most toxic. Long-term exposure to arsenic contaminants can cause cancer.
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