Hazardous Substance Residue

Hazardous Substance Residue

The Necessity of Detecting Hazardous Substances in Food by ELISA

The Necessity of Detecting Hazardous Substances in Food by ELISA

Food is an important material guarantee for human life and health. Contaminated food is bound to endanger human health. In addition to excessive veterinary drug residues, biotoxin residues, excessive pesticide residues, heavy metal contamination, bacteria, fungi, and parasite contamination, the factors that cause food contamination include some other harmful substances. These harmful substances may be chemical organic matter, self-active substances, and so on. Due to the frequent occurrence of food safety issues, the public is paying more and more attention to food safety. Ensuring food safety is an important public health issue. Because ELISA has the characteristics of simplicity, speed, high specificity, and sensitivity, it is widely used to detect harmful substances in food.

The Common Types of Hazardous Substances in Food That Can Be Detected by ELISA

Melamine is a chemical raw material that can be used in the production of plastics, coatings, adhesives, and food packaging materials. It is neither a food raw material nor a food additive, and it is forbidden to be added to food artificially. It should not appear in food or animal feed under normal circumstances. In addition to illegal addition, melamine contamination in food may also come from sources of pesticides, veterinary drugs, packaging sources, and so on. The target organs of melamine are the bladder and kidneys, which mainly cause cystitis, bladder stones, and kidney inflammation. Long-term high-dose melamine can induce bladder cancer in animals.

Histamine poisoning mainly occurs in coastal areas and areas where there is a habit of eating seafood. The high-histamine-containing fish are mainly the green-skinned red-flesh fish among the marine fish. When the fish body is not fresh or spoiled, the bacteria decompose the histidine in the fish body into histamine, which increases the histamine content, which can cause allergic food poisoning after ingestion. Histamine poisoning has an acute onset, mild symptoms, and quick recovery. The onset usually occurs 0.5 to 1 hour after eating. Mainly manifested as blushing, dizziness, palpitation, chest tightness, and shortness of breath. Some patients may have blurred vision, face swelling, lipedema, numbness of mouth, tongue, and limbs, nausea, vomiting, abdominal pain, urticaria, body flushing, etc.

Lean meat powder drugs are highly toxic, and many countries have banned the use of clenbuterol in food-borne animals. Lean meat meal can increase muscle protein content while reducing animal muscle fat tissue. However, such substances will remain in animal tissues, especially in internal organs such as the liver. After consumption, humans may experience symptoms such as muscle tremor, palpitation, trembling, headache, nausea, and vomiting, especially for high blood pressure and heart disease. Patients with diseases such as glaucoma, diabetes, hyperthyroidism, and prostate enlargement are more harmful and can lead to death in severe cases.

The Advantages of Using ELISA to Detect Hazardous Substances in Food

  • Can effectively avoid food safety problems
  • Can specifically detect harmful substances in food
  • Can provide effective monitoring methods for food safety

Creative Diagnostics has been committed to the research of detecting hazardous substances in food. We provide reliable ELISA kits to detect hazardous substances. Supported by rich related R&D experience and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and believable ELISA development services related to the detection of hazardous substances in food. If you wish a lot of careful data, please contact us.


  1. Yin, W.; et al. Preparation of monoclonal antibody for melamine and development of an indirect competitive ELISA for melamine detection in raw milk, milk powder, and animal feeds. J Agric Food Chem. 2010, 58(14): 8152-8157.
  2. Lieberman, P. The basics of histamine biology. Ann Allergy Asthma Immunol. 2011, 106(2 Suppl): S2-5.
  3. Ma, L.; et al. Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA. Food Chem. 2018, 246: 437-441.
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