Development of Cell-Based ELISA
Cell-based ELISA is a novel, rapid and sensitive qualitative protein detection technique. Cells are directly cultured in 96-well microplates, and protein changes in microplates can be measured directly without lysing cells. It can be used to monitor the activation of the state of certain biological pathways in cells. Creative Diagnostics provides you with high-quality cell-based ELISA kits and also develops new cell-based ELISA assays for your specific needs.
Why Choose Development of Cell-Based ELISA?
When you need a more sensitive and rapid ELISA method but the conventional ELISA format cannot meet your research needs, when you want to develop a new ELISA format that can accurately detect targets to accelerate your research progress, or when you want to develop a new protein-specific assay to gain a greater competitive advantage, you can choose the development of cell-based ELISA.
Questions to Consider Before Choosing This Service
- Do you need to measure specific molecules on the cell surface?
- Do you need to measure the phosphorylation ratio of a certain protein?
- Do you need to investigate the effect of stimuli/inhibitors on the target protein?
If your answer is yes, it means you can choose our cell-based ELISA development service. If you still have questions, we will provide you with a detailed consultation service to help you choose the appropriate ELISA type.
Advantages and Disadvantages of Cell-Based ELISA
In cell-based ELISA, cells are coated in 96-well plates or cell culture plates instead of soluble antigens, treated with stimulators or inhibitors, and primary antibodies are added for specific binding, then enzyme-labeled secondary antibodies are added for color development and determination.
Cell-based ELISA can be divided into dual-channel cell-based ELISA and single-channel cell-based ELISA. The former can detect two target proteins at a time but requires fluorescence detection methods and corresponding instruments, while the latter can only detect one target protein at a time.
The Main Advantages of Cell-Based ELISA
- Two different proteins can be detected simultaneously.
- Any surface molecule of adherent/non-adherent cells can be assayed.
- No protein extraction is required, errors are reduced, experimental procedures are simplified, and efficiency and sensitivity are improved.
The Main Disadvantages of Cell-Based ELISA
- The antigenic mass could not be determined.
- The requirements for the instrument are higher.
Fig. 1 The Flowchart of Cell-Based ELISA
Development of Cell-Based ELISA
Cell-Based ELISA involves cell culture and permeabilization treatment and has stricter operating requirements. The following is a brief description of the workflow for executing cell-based ELISA development services.
First of all, our cell-Based ELISA development team will evaluate your research and development purposes, and after confirming that this service is suitable for you, we will design a program based on your needs (target protein, sensitivity, reproducibility, etc.).
We will select suitable cells/or your specified cells to culture according to your requirements. Cell-based ELISA can detect adherent, semi-adherent, and suspension cells.
Cell-based ELISA involves primary and secondary antibodies. Based on years of experience, we provide complete services from gene synthesis to antibody preparation.
As with other ELISA formats, prepared antibodies need to be analyzed at the level of biomolecular interactions to ensure the success of the experiment.
In cell-Based ELISA, ALP β-gal is usually chosen to label the secondary antibody. We will use state-of-the-art technology to prepare enzyme-labeled antibodies.
In the development process of Cell-Based ELISA, in addition to selecting appropriate reagents and components, we also need to permeabilize cells.
After completing the development of the protocol, we will optimize the cell-based ELISA protocol according to the data feedback such as accuracy, assay range, and sensitivity, to provide you with the best cell-based ELISA kit.
After the optimization of the program is completed, we will also test and verify the cell suspension you specify to ensure that the program fully meets your individual needs.
After completing the above steps, we will produce the specified number of ready-to-use cell-based ELISA kits according to your requirements, and provide all related reagents (such as wash buffer, PBS buffer, PBS solution, etc.).
Result/Material Delivery
Development Protocol
All Experi-mental Data
Ready-to-use ELISA Kits
Validated Assay Results
Creative Diagnostics has established a scientific and efficient R&D system to provide you with the best cell-based ELISA development services. At the same time, we also provide corresponding ELISA testing services and provide comprehensive solutions for your ELISA kit customization needs. Please contact us directly for more information on the development of cell-based ELISA.
