In recent years, bioengineering technology has developed rapidly. Combining traditional ELISA with localized surface plasmon resonance (LSPR), a highly sensitive plasmonic ELISA based on nanomaterials was developed. As a leading biotechnology company, Creative Diagnostics can use our high-end technology platform to develop of plasmonic ELISA for your special needs.
When you are looking for a new enzyme-linked immunosorbent assay technology to overcome the shortcomings of the traditional ELISA in detecting some trace or trace analytes and improve the performance of ELISA, when you are developing a new ELISA format to better meet your needs for highly sensitive detection of analytical targets, you can choose our plasmonic ELISA development service.
If your answer is yes, then we are honored to provide you with plasmonic ELISA development services. If you still have some questions, we can also provide you with comprehensive answers to help you choose the most suitable ELISA development service.
Plasmonic ELISA is a novel assay technology that uses enzyme-catalyzed, mediated surface plasmon resonance (SPR) of AuNPs to generate solutions of different colors for visual reading.
Fig. 1 The schematic illustration of plasmonic ELISA based on enzyme induced aggregation of citrate-stabilized AuNPs. (Nie X, et al., 2014)
Plasmonic ELISA has many advantages over traditional ELISA, but the formation and growth kinetics of nanoparticles are strictly dependent on reducing agents, which greatly hinders the practical application of plasmonic ELISA. In order to meet your needs and facilitate the popularization of plasmonic ELISA, we strictly implement the workflow of the plasmonic ELISA development service.
Our Plasmonic ELISA development team will design the best development strategy based on your various needs for plasmonic ELISA. At present, there are four main development strategies in different directions, namely:
We will prepare corresponding high-standard antigens according to your analytical target.
Consistent with the development of traditional ELISA, antibody preparation was also involved in the development of plasmonic ELISA. We will prepare the required primary and secondary antibodies according to different strategies.
Antibody analysis is an indispensable step. The level of biomolecular interaction of antibodies is critical for the successful development of new plasmonic ELISA assays.
In the development of plasmonic ELISA, we also need to label the secondary antibody. Biotin is our best choice for labeling secondary antibodies.
In plasmonic ELISA, the color change occurs due to the control of the nanoparticle formation and growth kinetics by the reducing agent. The selection of reducing agents is one of the most critical links in the development of plasmonic ELISA. Based on different nanoparticles, we can choose from the following reducing agents:
The LSPR properties of plasmonic metal nanoparticles are the cornerstone of plasmonic ELISA. In this step, we need to select stabilizers to prepare stable metal nanoparticles. We usually choose to use biotinylated acetylcholinesterase and citrate as stabilizers, and nanoparticles are usually AuNPs and AgNPRs.
After doing the above preparations, we will select various reagents and components needed in the plasmonic ELISA, and proceed to the development of the plasmonic ELISA protocol.
The synthesis and growth of metal nanoparticles are very sensitive to reaction conditions and reagent concentrations, so we will rationally optimize the development protocol based on the feedback of detection data.
After the optimization is complete, we will carry out the assay verification of your biological sample to ensure that you are provided with the correct plasmonic ELISA development protocol.
After completing all the procedures, with your permission, we will manufacture the specified plasmonic ELISA kits containing all the reagents for you.
The new era of nano-ELISA has long arrived, but it is not widely used due to its limitations. Creative Diagnostics will make every effort to provide you with a high-quality plasmonic ELISA assay development protocol. At the same time, we also provide corresponding ELISA testing services and comprehensive solutions for your customized ELISA kit needs. Please feel free to contact us if you need to develop a plasmonic ELISA.