Polycyclic Aromatic Hydrocarbons

Polycyclic Aromatic Hydrocarbons

The Severity of Environmental Contamination Caused by Polycyclic Aromatic Hydrocarbons

Polycyclic Aromatic Hydrocarbons Testing

Polycyclic aromatic hydrocarbons (PAHs) refer to aromatic hydrocarbons containing two or more benzene rings. They are a type of persistent organic contaminants and have strong carcinogenic, teratogenic, and mutagenic properties. PAHs have a wide range of pollution sources. Incomplete combustion of organic materials such as coal, petroleum, wood, tobacco, and organic polymer compounds will produce PAHs. PAHs widely exist in the atmosphere, soil, water, sediment and other environmental media. PAHs are semi-volatile and can cause global pollution. The analysis of the atmospheric composition of eight continents in the United States shows that the PAHs in the atmosphere in industrial areas are more than 10 times higher than in agricultural areas. Moreover, PAHs can continuously accumulate, posing potential threats to the ecosystem and human health through the food chain.

The Necessity of Monitoring Polycyclic Aromatic Hydrocarbons with ELISA Testing

Fig. 1 The chemical structure of biphenyleneFig 1. The chemical structure of biphenylene

PAHs are genetically toxic, mutagenic and carcinogenic, and can cause many harms to the human body, such as damage to the respiratory system, circulatory system, and nervous system, and can also damage the liver and kidneys. As the number of rings increases, the chemical structure changes, and the hydrophobicity increases, its electrochemical stability, durability, resistance to biodegradation, and carcinogenicity will increase. As people pay more and more attention to environmental pollution, the monitoring of PAHs is becoming more and more important. The most common method currently used is ELISA, which has the advantages of simple operation, high sensitivity, strong specificity and low analysis cost. It is currently one of the ideal methods for detecting PAHs contaminants.


Direct ELISA

The Advantages of ELISA Testing

  • Can detect PAHs in soil and water quickly and at a low cost
  • Capable of high-throughput analysis of soil and water samples to be tested
  • The sample to be tested only needs simple processing such as extraction and dilution

ELISA Procedure for Polycyclic Aromatic Hydrocarbons Testing

Firstly, the microtiter plates were coated with 500 µL anti-PAH antibody.
250 µL/well PAH assay or 200 µL C-PAH assay of the diluted sample extracts or the standard solutions were added to the plate, vortexing and further incubated for 30 min at room temperature.
After washing twice with the washing solution, 500 µL of the color solution was added.
Terminated by adding 500 μL of stopping solution and incubated for 20 min at room temperature. The absorbance was read at 450 nm by a microplate reader.

Creative Diagnostics has been committed to organic contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of polycyclic aromatic hydrocarbons. If you wish a lot of careful data, please contact us.


  1. Kim, K.H.; et al. A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health effects. Environ Int. 2013, 60: 71-80.
  2. Chuang, J.C.; et al. Evaluation of enzyme-linked immunosorbent assay for the determination of polycyclic aromatic hydrocarbons in house dust and residential soil. Sci Total Environ. 1998, 224 (1-3): 189-199.
  3. Meng, X.Y.; et al. An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons. Anal Biochem. 2015, 473: 1-6.

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