Development of Paper-Based ELISA

Development of Paper-Based ELISA

ELISA is one of the commonly used immunological assay methods. With the rapid development of material science, more and more factors are applied to ELISA. Due to the superiority of paper, the researchers used it to replace the traditional 96-well polystyrene plate and established a paper-based ELISA. As a front-line ELISA research team, Creative Diagnostics can provide paper-based ELISA development services to meet your multiple needs.

Why Choose Development of Paper-Based ELISA?

ELISA. Paper has a wide range of sources and low cost. The liquid can flow without external force through capillary action, and it has good biocompatibility and is easy to carry. If you want to develop new ELISA analysis technology with the above advantages, then you can choose our paper-based ELISA development service.

Questions to Consider Before Choosing This Service

  1. Do you need to develop a paper-based ELISA format?
  2. Do you need to perform ELISA detection on the target without equipment?

If your answer is yes, then you can choose us to develop a paper-based ELISA protocol for you. If you have any ideas, you can contact us directly to discuss and choose the most suitable ELISA development service for you.

Advantages and Disadvantages of Paper-Based ELISA

The basic principle of paper-based ELISA is the same as that of traditional ELISA, except that it uses paper as solid support.

The Main Advantages of Paper-Based ELISA

  • Low cost, paper as solid support reduces costs.
  • Easier, use a desktop scanner to analyze the test results.
  • Low sample volume, requiring less than 20 mL of reagent for an assay.
  • Faster, a complete paper-based ELISA assay can be performed in less than an hour.

The Main Disadvantage of Paper-Based ELISA

  • Low sensitivity, the immobilization of antibodies in paper-based ELISA is susceptible to pH, temperature, and other factors, and about 40% of the antibodies will dissociate during the washing process.

The schematic illustration of paper-based ELISA for E. coliFig. 1 The schematic illustration of paper-based ELISA for E. coli. (Shih C, et al., 2015)

Development of Paper-Based ELISA

Although paper-based ELISA has many advantages, its low sensitivity deserves our attention. To provide high-quality paper-based ELISA development services, we will strictly comply with industry regulations and implement the following workflow.

The first step in the development of paper-based ELISA is to design a development protocol according to your individual needs.

From gene optimization and construct design to antigen synthesis, we have a professional team to serve you. We will prepare the best quality antigen for the next step of antibody preparation according to your needs.

As far as antibody preparation is concerned, we have many years of experience and advanced technology and equipment. Whether monoclonal or polyclonal, our antibody preparation technology platform can synthesize antibodies that meet your needs.

After the antibodies are prepared, the level of biomolecular interaction is routinely measured.

In a paper-based ELISA, we need to label the secondary antibody.

In paper-based ELISA, the selection of paper carriers is one of the most important steps. Commonly used paper carriers are nitrocellulose membrane, filter paper, chromatography paper, common printing paper, and so on. Each paper carrier has different characteristics, and we choose the appropriate paper carrier according to the characteristics of the target.

After the paper carrier is selected, micropores need to be prepared on the paper carrier. Micropores are usually prepared by photolithography, wax printing, printing, stamping, and other methods.

After the preparations are completed, we will proceed to the development of the paper-based ELISA according to the established protocol.

emperature and pH in Paper-based ELISA are the influencing factors that need to be focused on. Based on the preliminary assay data, we need to optimize it as well as the paper-based carrier to provide you with the best quality paper-based ELISA development solution.

Validation of the assay for the development protocol is an essential step. We will perform a paper-based ELISA assay on your specified biological samples to ensure that the recombinant proteins/peptides detected are consistent with your target targets.

When the development protocol has been validated as correct, we will produce the specified paper-based ELISA kits according to your requirements.

Result/Material Delivery

Development Protocol

Development Protocol

All Experi-mental Data

All Experi-mental Data

Ready-to-use ELISA Kits

Ready-to-use ELISA Kits

Validated Assay Results

Validated Assay Results

Creative Diagnostics provides precise paper-based ELISA development services according to your needs. We also provide corresponding ELISA testing services and comprehensive solutions for your ELISA kit customization needs. If you are interested in our paper-based ELISA development services, please contact us directly.


  1. Shih C, et al. (2015). "Paper-based ELISA to rapidly detect Escherichia coli." Talanta. 145, 2–5.
The service is for research only, not for clinical use.
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