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The Severity of Environmental Contamination Caused by Polychlorinated Biphenyls

Polychlorinated Biphenyls Testing

Polyc hlorinated biphenyls (PCBs), also known as polychlorodiphenyls, are a type of chlorinated hydrocarbon compounds produced by high-temperature chlorination of biphenyl as raw material under the action of a metal catalyst. Polychlorinated biphenyls were once widely used in industry. Although they are currently banned, the discharge of waste since their production has caused global environmental pollution problems. Around 1944, PCBs obviously polluted the ecosystem, and the pollution range was very wide. PCBs can be detected from seals in the Arctic to seabird eggs in the Antarctic. It is estimated that the total amount of PCBs in the world's oceans, soils, and atmosphere reaches more than 250,000 to 300,000 tons. PCBs are persistent contaminants and are difficult to degrade, and the water and soil contaminated by them are also difficult to recover.

The Necessity of Monitoring Polychlorinated Biphenyls with ELISA Testing

Fig 1. The chemical structure of biphenyl

PCBs in water bodies will reduce the diversity of aquatic organisms, can accumulate in the body, and then endanger human health through the food chain. PCBs can be absorbed by the human body through the skin, respiratory tract, and digestive tract. And accumulate in adipose tissue, causing diseases of the brain, skin and internal organs, and affecting the nervous, reproductive and immune systems. The International Agency for Research on Cancer of the World Health Organization has listed PCBs as a class of carcinogens. Because of its carcinogenicity, reproductive toxicity, neurotoxicity, and endocrine system interference, it has become one of the toxic contaminants monitored by many countries. ELISA has been widely used in the detection of PCBs as a highly sensitive, specific, and convenient detection method.

ELISA Type

Direct Competitive ELISA

The Advantages of ELISA Testing

Convenient to monitor PCBs in the environment
Can specifically detect PCBs in environmental samples
High-throughput detection of PCBs in environmental samples
Provide a faster, simpler and more cost-effective method for detecting PCBs

ELISA Procedure for Polychlorinated Biphenyls Testing

The microtiter plates were coated with 200 µL sheep polyclonal antibody diluted in coating buffer (1:40,000), and incubated overnight at room temperature

After washing three times with PBST, 100 μL/well of Delor 103 standards or soil extracts diluted 1:100 with assay solution were added.

Then 50 µL/well PCB-peroxidase conjugate diluted 1:400,000 with PBS was added, shaken for 1 min followed by incubation for 1 h at room temperature.

After washing three times with PBST, 200 µL enzyme substrate (H₂O₂ and TMB solution) was added and shaken for 15 min.

Terminated by adding 50 μL of 2M H₂SO₄, and the absorbance was read at 450 nm with an ELISA reader.

Creative Diagnostics has been committed to organic contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of polychlorinated biphenyls. If you wish a lot of careful data, please contact us.

References

Rodenburg, L.A.; et al. Evidence for unique and ubiquitous environmental sources of 3,3'-dichlorobiphenyl (PCB 11). Environ Sci Technol. 2010, 44(8): 2816-2821.
Liu, J.; et al. A Critical Review of Polychlorinated Biphenyls Metabolism, Metabolites, and Their Correlation with Oxidative Stress. Chem Res Toxicol. 2020, 33(8): 2022-2042.
Deng, A.P.; et al. Direct competitive ELISA for the determination of polychlorinated biphenyls in soil samples. Anal Bioanal Chem. 2002, 373(8): 685-690.

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