Lean Meat Powder

Lean Meat Powder

The Harm Caused by Lean Meat Powder-Contaminated Food

The Harm Caused by Lean Meat Powder-Contaminated Food

Lean meat meal is a general term for phenylethanolamine compounds that can promote protein synthesis and inhibit fat deposition. This class of compounds belongs to the adrenergic receptor agonist class and can be generally divided into three categories aniline type, phenol type, and hydroquinone type according to their structure. The aniline type includes clenbuterol, cimaterol, mabuterol, clenbuterol, etc.; the phenol type includes ractopamine, salbutamol, and salbutamol sulfate; the hydroquinone type includes dopamine hydrochloride, chlorpromazine hydrochloride, terbutaline sulfate. In recent years, new lean meat powders such as clonidine, baclofen, and cyproheptadine have appeared. After feeding a lean meat meal, animal muscle content increases, and it is more likely to be favored by consumers. However, such substances will remain in animal tissues, especially in internal organs such as the liver. After human consumption, symptoms such as muscle tremor, palpitation, trembling, headache, nausea, and vomiting can occur, especially for patients with diseases such as hypertension, heart disease, hyperthyroidism, and prostatic hypertrophy, and severe cases can lead to death.

The Necessity of Monitoring the Contamination of Lean Meat Powder in Food by ELISA

The Necessity of Monitoring the Contamination of Lean Meat Powder in Food by ELISAFig. 1 The chemical structure of Clenbuterol

The frequent occurrence of poisoning incidents caused by the consumption of food contaminated by "Clenbuterol" has attracted the attention of countries all over the world. In order to ensure the quality and safety of livestock products and protect human health, many countries have banned the use of clenbuterol in the production of food-borne animals. Residues are mandatory inspection items, and the EU also prohibits the addition of "clenbuterol" drugs in feed. However, some unscrupulous businessmen add lean meat meals to the feed for profit, posing a health threat to the public. To combat this kind of behavior, we should strictly monitor the substance. Because ELISA is accurate, sensitive, and simple, it has now become a widely used method for detecting lean meat powder.

ELISA Types

Indirect competitive ELISA, Paper-based competitive ELISA

The Advantages of ELISA Testing

  • High sensitivity, can accurately detect lean meat powder substances
  • Low testing cost, easy operation, no professional restriction on testing personnel
  • Rapid detection, can quickly detect whether lean meat powder is added

ELISA Procedure for Clenbuterol Testing

1
Spot the mouse monoclonal clenbuterol antibody on each microzone, and then place it at room temperature to dry completely.
2
After washing three times with 10 μL washing buffer, add blocking buffer to each microzone to avoid non-specific binding of the target analyte.
3
After diluting the sample 5 times with 1×PBS, pipette 3 μL to the microzone and incubate at 22°C for 2 min.
4
Add 3 μL of HRP-labeled clenbuterol to each microzone, incubate for 5 minutes and rinse the microzone three times.
5
Finally, a total of 5 μL of TMB spots are placed in each microzone, and the camera takes a picture of the microzones after 2 minutes of reaction.

Creative Diagnostics has been committed to hazardous substances testing by ELISA. We provide reliable ELISA kits for the detection of lean meat powder. Supported by rich professional knowledge, we provide high-quality customized ELISA kits services, professional ELISA testing services, and believable ELISA development services related to lean meat powder. If you wish a lot of careful data, please contact us.

Reference

  1. Ma, L.; et al. Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA. Food Chem. 2018, 246: 437-441.

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