Products

Dioxins

The Severity of Environmental Contamination Caused by Dioxins

Dioxins Testing

Dioxins usually refer to a group of polychlorinated substituted planar aromatic compounds with similar structures and physical and chemical properties, including polychlorinated dibenzodioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs). Among them, 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is by far the most toxic pollutant known to mankind. Dioxins are a group of chemical substances with persistent pollution to the environment. The sources of dioxins in the atmospheric environment are complex. The emissions from steel smelting, incineration industry, the use of defoliants, the preparation of pesticides, and automobile exhaust emissions are the main sources of dioxins in the environment. Waste incineration has received the highest degree of attention. Dioxins are distributed globally and have been found in almost all media in the world. The most serious places where these compounds accumulate are in the soil, sediment and food.

The Necessity of Monitoring Dioxins with ELISA Testing

Fig. 1 The chemical structure of tetrachlorodibenzodioxin Fig 1. The chemical structure of tetrachlorodibenzodioxin

Human short-term exposure to high doses of dioxins may cause skin damage and may also alter liver function. Long-term exposure can damage the immune system, the developing nervous system, the endocrine system, and reproduction. Studies have pointed out that dioxins may also cause poor fetal growth. Chronic exposure to dioxins in animals has caused several types of cancer. The WHO International Cancer Institute classifies TCDD as a "known human carcinogen". Dioxins in the environment can also be enriched and transmitted through the food chain, endangering human health. Due to the high potential toxicity of these compounds, long-term monitoring of their concentration in the environment is required. ELISA is used in the detection of dioxin because of its convenience, speed and high sensitivity.

ELISA Type

Indirect ELISA

The Advantages of ELISA Testing

Dioxins can be tested quickly and at a low cost
Can simplify sample preparation procedures
High-throughput detection and analysis of dioxin contamination

ELISA Procedure for Dioxins Testing

The microtiter plates were coated with III-BSA antigen (0.05 μg/mL in coating buffer, 100 μL/well), and incubated overnight at 4°C.

After washing with PBST, 200 μL/well of blocking solution (0.5% BSA in PBS) was added, and incubated for 30 min at RT.

After washing, 50 μL/well of antibody 7598 (1/3500 in PBS with 0.2% BSA) and 50 μL/well of sample or standard (either TMDD or TCDD) (prepared in DMSO containing 0.01% Triton X-100: PBS, 1:1, v/v) were added, incubated for 90 min at RT.

After washing five times with PBST, 100 μL/well of goat anti-rabbit IgG conjugated to HPR was added, incubated for 60 min at RT.

After washing five times with PBST, the substrate solution was added (100 μL/well; 3.3 μL of 30% H₂O₂, 400 μL of 0.6% tetramethylbenzidine in DMSO per 25 mL of citrate–acetate buffer, pH 5.5), incubated for 10-20 min at RT.

Terminated by adding 50 μL/well of 2M H₂SO₄, and the absorbance was read at 450 nm by a microplate reader.

Creative Diagnostics has been committed to organic contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of dioxins. If you wish a lot of careful data, please contact us.

References

Nichkova, M.; et al. Immunochemical determination of dioxins in sediment and serum samples. Talanta. 2004, 63(5): 1213-1223.
Emon, J.M.; et al. An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples. Chemosphere. 2008, 72(1): 95-103.

Online Inquiry

Navigation