Stable Cell Line Development Service
Gene expression systems fall into two main categories: transient expression and stable expression. Transient expression involves introducing your gene of interest into cells via plasmid or viral delivery, producing high protein levels for a short period (typically 7–10 days). It's ideal for quick functional screens and preliminary protein production, but the gene is gradually lost as cells divide. In contrast, stable expression permanently integrates the gene into the host genome, ensuring long-term, uniform expression—perfect for extended experiments, drug screening, and large-scale production.
Stable cell line development relies on lentiviral vectors' ability to integrate into the genome. Creative Diagnostics packages your gene into lentivirus, infect host cells, and use selectable markers (antibiotic resistance or fluorescence) to isolate and expand clones with stable genomic integration. Rigorous screening and validation yield genetically uniform cell lines with consistent expression, meeting the demands of both research and production.
Our Services
- Lentiviral Packaging & Purification
We generate high-titer lentivirus carrying your gene of interest and purify it to ensure safe, efficient delivery. - Stable Cell Line Selection
We tailor the selection strategy to your project needs—antibiotic resistance, fluorescent markers, or both—to quickly identify cell populations with stable gene expression. - Validation & Expansion
We confirm expression via antibiotic assays, fluorescence imaging, qPCR, and Western blot, then scale up and cryopreserve your stable cell lines. - Technical Documentation & QC Reports
You receive a comprehensive dossier including plasmid maps, viral titer reports, selection protocols, and validation data.
Key Strengths
Our stable cell line development combines the efficiency of lentiviral integration with flexible selection markers to suit any mammalian cell type, even those that are hard to transfect. A stringent quality-control pipeline from virus production through clone validation guarantees cell lines that deliver consistent expression, genetic stability, and reproducible results.
Workflow
Provide your gene name, species, vector preferences (resistance marker, fluorescent tag, etc.), host cell line, and any special requirements.
We perform small-scale tests to verify plasmid expression and complete lentiviral packaging and titering.
We choose the optimal selection method based on your specifications to balance efficiency and expression quality.
Expression is verified by antibiotic selection, fluorescence imaging, qPCR, and Western blot. Stable clones are expanded and cryopreserved.
You receive your cell lines along with plasmid maps, viral titer reports, and validation data, plus ongoing technical support as needed.
Applications
Protein function and interaction studies
Signaling pathway and epigenetics research
Drug screening and target validation
High-yield recombinant protein or antibody production
Subcellular localization and imaging experiments
Notes
- This service is limited to mammalian cell lines; we do not handle plant, bacterial, or fungal systems.
- Clients must supply legally compliant cell lines and disclose any ethical or safety approvals required.
- Gene characteristics (e.g., toxicity, expression level) can affect selection efficiency—please discuss specifics with our technical team.
- Single-clone selection can be time- and cost-intensive; we'll recommend the best approach based on your project goals.
We look forward to partnering with you to build high-quality stable cell lines and advance your research and development!
