Cell Apoptosis Detection
Apoptosis, or programmed cell death, is essential for tissue homeostasis and development. Dysregulated apoptosis is linked to diseases such as cancer, autoimmune disorders, and neurodegeneration. Accurately and efficiently measuring apoptosis levels is critical for studying signaling pathways, screening anti- or pro-apoptotic compounds, and investigating gene function.
Our Service
Creative Diagnostics uses the Annexin V/PI dual-staining method combined with flow cytometry to precisely quantify cells at different stages of apoptosis:
- Principle: In early apoptosis, phosphatidylserine (PS) flips from the inner to the outer leaflet of the plasma membrane. Fluorescent Annexin V binds exposed PS, while propidium iodide (PI) only penetrates cells with compromised membranes (late apoptotic or necrotic). Dual-channel flow cytometry distinguishes and quantifies live cells, early apoptotic cells, and late apoptotic/necrotic cells.
- Purpose: To differentiate and quantify each apoptosis stage, evaluating the effects of treatments or genetic interventions.
- Materials: Annexin V reagent, PI staining solution, binding buffer, PBS, and flow cytometry tubes.
- Steps:
- Prepare cells;
- Harvest and wash cells;
- Stain with Annexin V and PI;
- Acquire data by flow cytometry;
- Analyze results and generate a report.
Key Advantages
Our experienced cell biology team and high-performance flow cytometers ensure rigorous quality control from sample preparation and staining to data analysis. We deliver sensitive, accurate, and reproducible results across various cell types, including primary cells, cancer cells, and stem cells.
Workflow
Discuss cell source, treatment conditions, and assay requirements.
Tailor staining and detection parameters to your samples.
Perform cell preparation, collection, washing, and Annexin V/PI staining.
Collect flow cytometry data and quantify apoptosis stages.
Provide a detailed results report with charts and offer recommendations for optimization.
Notes
- Sample quality (viability, contamination) directly affects results; please confirm details in advance.
- Perform staining and detection under light-protected conditions to prevent fluorescent signal loss.
- Optimize flow cytometer settings according to cell type and probe characteristics.
We look forward to delivering professional, reliable apoptosis assays to support your research and drug development efforts!
