Colony Formation Assay

The colony formation assay evaluates a single cell's ability to survive and proliferate independently, with colony formation rate reflecting population dependence and proliferative potential. This method is widely used in tumor biology, gene function studies, and anticancer drug screening.

Our Services

Creative Diagnostics offers a comprehensive, one-stop colony formation assay service:

• Principle
  Single-cell suspensions are plated and cultured continuously until visible colonies form. Comparing colony counts between treatment and control groups reveals how gene overexpression or knockdown affects cell survival and proliferation.
• Purpose
  Quantitatively compare colony numbers in gene-modified cells, vector-only controls, and negative controls to assess the gene's impact on population dependence and growth capacity.
• Materials
  Log-phase cells (treatment and control groups)
  Culture dishes
  Culture medium and required reagents
  Fixation and staining solutions
  PBS buffer
• Procedure
  1. Prepare and single-cell suspension
  2. Plate cells at low density
  3. Culture until colonies form
  4. Fix and stain colonies
  5. Count colonies and analyze data

Key Advantages

With rigorous experimental design and extensive experience, we optimize seeding density and culture time for each cell type, ensuring even colony distribution and accurate results. Automated image analysis software enhances throughput and consistency.

Workflow

Notes

• Optimize seeding density to avoid overlapping or too few colonies.
• Adjust culture duration based on cell characteristics.
• Include proper controls to eliminate non-specific effects.
• Follow biosafety and waste-disposal regulations.

We look forward to providing you with a professional, efficient colony formation assay to support your gene function research and anticancer drug evaluation!