Services
Flow Cytometry Panel Design Service
Panel design is a critical step in flow cytometry experiments. Proper fluorochrome selection maximizes signal resolution, minimizes compensation complexity, and ensures accurate, reproducible data. Creative Diagnostics offers expert panel design services tailored to your experimental goals and instrument setup, empowering your research for efficient and reliable results.
Services Offered
| Phase | Key Activities |
| Requirement Gathering | - Collect experimental details: target antigen expression levels, co-expression or exclusion relationships, and relevant literature references. - Review your flow cytometer configuration: laser lines, detection channels, and filter sets. |
| Panel Design | - Channel matching: identify compatible laser and filter combinations. - Fluorochrome selection: use spectral databases to choose dyes with minimal overlap and appropriate brightness. - Expression assessment: assign high-brightness dyes to low-abundance markers and vice versa. - Compensation planning: anticipate spectral spillover and recommend compensation settings to reduce downstream workload. |
| Validation & Optimization | - Literature and database verification: confirm dye characteristics using authoritative publications and manufacturer data. - Expert review: senior flow cytometry specialists evaluate and refine the panel. - Client feedback: incorporate your input to finalize a practical, high-performance panel. |
| Ongoing Support | - Experimental guidance: sample preparation, antibody titration, and instrument setup tips. - Data analysis consultation: optional assistance with initial data processing and interpretation. - Technical support: 7×12-hour online help for troubleshooting and questions. |
Why Choose Us
- Customized Solutions: Tailored to your specific markers and cytometer configuration for an optimal fit.
- Expertise You Can Trust: Our team has years of hands-on experience developing and applying multicolor panels.
- Time Savings: From design through guidance, we streamline the process so you can focus on generating data.
Typical Applications
- Immunophenotyping: Subset analysis of lymphocytes or tumor-infiltrating immune cells.
- Functional Assays: Proliferation (Ki-67), apoptosis (Annexin V/PI), reactive oxygen species probes, and more.
- Rare Cell Detection: Circulating tumor cells, hematopoietic stem cells, and other low-frequency populations.
- High-Parameter Panels: Eight-, ten-, or higher-color panels for complex phenotyping studies.
The service is for research only, not for clinical use.
