Sialylation Modification Omics Analysis
Sialylation, as a crucial step in glycosylation modification, refers to the process of adding sialic acid monosaccharides to the terminal ends of glycan chains or oligosaccharides in glycoconjugates. This modification is one of the most common post-translational modifications of proteins, widely found at the terminals of cell surface glycoproteins and plays a key role in various biological activities.
Recent studies have revealed that sialylation regulates protein-protein interactions, intracellular localization, vesicle transport, and signal transduction, performing essential functions in organisms, including embryonic development, neural development, somatic reprogramming, and cancer progression. Abnormal sialylation modifications are closely associated with various disease states, including cancer, neurodegenerative diseases (such as Parkinson's and Alzheimer's), and autoimmune disorders.
Our Services
Our sialylation modification omics analysis service offers comprehensive solutions, including:
- Sialic Acid Content Analysis: Precise detection of the content and ratio of sialic acids (Neu5Ac and Neu5Gc) in samples.
- Sialylation Site Identification: Determination of specific sialylation sites on glycoproteins using high-precision mass spectrometry.
- Sialylation Level Quantification: Comparative analysis of differential expression of sialylation modifications across samples.
- Intact Glycoprotein Structure Analysis: Investigation of the impact of sialylation on the overall structure and stability of proteins.
- Sialic Acid Oxidation Pathway Analysis: Analysis of the unique oxidation patterns of sialic acids under free radical oxidation conditions.
- Comprehensive Glycosylation Performance Analysis: Includes monosaccharide quantification, primary structure analysis of N- and O-glycans, and detailed full-structure sequencing of glycans.
Service Advantages
Our service platform is built on advanced analytical technologies, enabling cross-scale analysis from sialic acid molecular oxidation pathways to the conformational stability of intact glycoproteins. With extensive project experience and mature, stable technical processes, we ensure strict quality control at every analytical step, delivering reliable data quality and analytical results. Additionally, we prioritize service efficiency and are committed to providing responsive technical support and professional data interpretation services.
Workflow
Engage with clients to understand their needs, clarify analytical objectives, and provide technical consultation and solution recommendations.
Develop customized analytical plans based on project requirements, defining technical pathways and experimental designs.
Guide clients through sample preparation, offering professional advice on sample preservation and transportation to ensure sample quality meets analytical standards.
Execute standardized experimental procedures, including sample preparation, enzymatic digestion, and peptide enrichment.
Perform mass spectrometry data acquisition with stringent quality control measures.
Conduct bioinformatics analysis, including data mining and statistical validation.
Provide comprehensive technical reports, including experimental methods, analytical results, and research recommendations.
Sample Submission Guidelines
To ensure the accuracy of experimental results, please refer to the following sample submission requirements:
| Sample Type | Recommended Amount | Pretreatment Requirements |
| Animal Tissue | 200 mg | Fresh frozen or RNAlater preserved, dry ice shipping |
| Cells | 4×10⁷ cells | Centrifuged, PBS-washed, dry ice shipping |
| Serum, Plasma | 100 μL | Avoid repeated freeze-thaw cycles, dry ice shipping |
| Protein Solution | 4 mg | Indicate concentration and buffer composition, dry ice shipping |
Application
Our sialylation modification omics services are widely applicable in the following fields:
- Disease Mechanism Research: Elucidate the role of sialylation in cancer, neurodegenerative diseases, and autoimmune disorders.
- Biomarker Discovery: Identify abnormal sialylation patterns associated with diseases as potential diagnostic markers.
- Drug Development Optimization: Enhance the stability of protein drugs in complex physiological environments by modulating protein sialylation.
- Stem Cell and Regenerative Medicine: Investigate the role of sialylation in the establishment and maintenance of stem cell pluripotency.
- Immunoregulation Research: Explore the regulatory role of sialylation in immune recognition and immune responses.
- Mechanism of Drug Action Analysis: Reveal the impact of natural products, functional foods, and drug interventions on sialylation modifications.
As a cutting-edge research field, sialylation modification omics is increasingly becoming a key to understanding life processes and disease mechanisms. Our professional service team, leveraging advanced platforms and extensive experience, is dedicated to providing comprehensive, accurate, and efficient sialylation analysis services to support your scientific research and drug development.
