Kynurenine Targeted Metabolomics Analysis
In the forefront of life sciences, metabolomics has become a core tool for deciphering disease mechanisms and discovering biomarkers. Among its branches, targeted metabolomics, with its advantages of high sensitivity, high specificity, and precise quantification, plays an irreplaceable role in translational medical research. The kynurenine (Kyn) pathway, as the major branch of tryptophan metabolism, has been widely confirmed in recent years to participate in key physiological and pathological processes such as immune regulation, neurological function, and tumorigenesis. Research indicates that dysregulation of this metabolic pathway is closely associated with various major diseases including rheumatoid arthritis, cancer, and neurological disorders.
Addressing this cutting-edge target, we have developed a Precise Quantification Service for Targeted Metabolomics of the Kynurenine Pathway based on an advanced LC-MS/MS technology platform. This service enables absolute quantitative analysis of 7 core kynurenine-class metabolites in various biological samples such as serum, plasma, urine, cells, and tissues, providing reliable data support for your scientific research and clinical translation studies.
Our Services
Creative Diagnostics focuses on the in-depth analysis of the tryptophan-kynurenine metabolic pathway, offering a one-stop solution from sample testing to data analysis.
1. Core Detection Panel
Our detection panel covers 7 key metabolites of the kynurenine metabolic pathway, ensuring a comprehensive assessment of the pathway. Detectable metabolites include: Kynurenine (Kyn), Tryptophan (Trp), 3-Hydroxykynurenine (3-OH-Kyn), Xanthurenic acid (XA), Quinolinic acid (QA), Kynurenic acid (KA), and 5-Hydroxytryptamine (5-HT). By analyzing the absolute concentrations and mutual ratios (e.g., Kyn/Trp) of these metabolites, the overall activity and branch flow of the pathway can be precisely evaluated.
2. Advanced Technology Platform
This service is based on an UPLC-MS/MS platform using Multiple Reaction Monitoring (MRM) mode for detection. This method combines the high separation capability of chromatography with the high sensitivity and specificity of mass spectrometry, making it the gold standard for quantifying low-abundance metabolites in complex biological matrices. By optimizing chromatographic conditions and mass spectrometry parameters, and employing stable isotope-labeled internal standards for correction, we ensure quantification accuracy and reproducibility.
3. Support for a Wide Range of Sample Types
Our method, optimized and validated, is suitable for various biological sample matrices, including:
- Body Fluid Samples: Serum, plasma, urine, cerebrospinal fluid, etc.
- Tissue Samples: Animal tissues (e.g., liver, kidney, brain), plant tissues, etc.
- Cell Samples: Cultured cell pellets.
- Microbial Samples: Bacterial pellets.
We provide detailed guidelines for sample collection, pre-processing, and shipping to ensure sample quality and the reliability of subsequent test results.
Workflow
Our service process is clear, transparent, and features smooth communication, designed to provide you with an efficient and worry-free research experience.
You will discuss your research objectives, sample conditions, and testing needs in detail with our technical experts. We will tailor the optimal testing and analysis plan for you.
Both parties confirm the plan details and sign the service contract. You then process and arrange shipment of the samples according to our provided "Sample Preparation Guide".
Upon arrival at the laboratory, samples are registered, pre-processed, and analyzed on the LC-MS/MS platform. Strict quality control is implemented throughout the entire process.
The acquired raw mass spectrometry data undergoes integration, quantitative calculation, and professional statistical analysis and visualization.
We deliver a complete report containing detailed experimental procedures, raw data, analysis results, and preliminary interpretation. Our technical support team will continue to address your data-related questions until the successful conclusion of your project.
Applications
Precise quantitative analysis of the kynurenine pathway holds significant value in multiple life science research fields:
- Research on Mechanisms of Immune-related Diseases: Studies show that dysregulation of the kynurenine pathway is closely related to the pathogenesis and progression of autoimmune diseases such as Rheumatoid Arthritis (RA). Detecting levels of metabolites like tryptophan and kynurenine in serum can help assess the immune imbalance state in patients and provide a new metabolic dimension for disease stratification and treatment efficacy monitoring.
- Tumor Metabolism & Immune Microenvironment: In various cancers (e.g., head and neck squamous cell carcinoma), tumor cells can utilize the kynurenine pathway to mediate immune escape. Detecting kynurenine levels in tumor tissue or peripheral blood helps elucidate tumor immune suppression mechanisms and may provide potential biomarkers or combination therapy targets for immunotherapy.
- Neurological Disease Research: Metabolites of the kynurenine pathway (e.g., quinolinic acid) possess neuroactive properties and are implicated in pathological processes of neurodegenerative diseases and depression. This service can be used to explore the metabolic etiology of neurological disorders.
- Drug Development & Pharmacodynamic Evaluation: In the development of new drugs targeting the kynurenine pathway (e.g., IDO/TDO inhibitors), this service can serve as a crucial pharmacodynamic biomarker detection method for dynamically monitoring the inhibitory effect of drugs on the target pathway, accelerating preclinical and clinical research progress.
- Nutrition & Health Research: Assessing the state of tryptophan metabolism in relation to overall health and gut microbiota function.
If you are engaged in frontier exploration in related fields, please feel free to contact us for detailed technical information and service proposals. Let's use precise data to jointly advance your research to greater depth.
