Comprehensive Histone Modification Quantification Analysis
Histone modification represents one of the most crucial and versatile regulatory mechanisms in epigenetics. By adding chemical modifications such as acetylation and methylation to histone tails, it precisely controls gene switching on and off, playing a key role in vital physiological and pathological processes including development, cellular differentiation, tumorigenesis, neuropsychiatric disorders, and aging. Importantly, enzymes related to histone modifications have emerged as significant drug targets, with corresponding inhibitors already being used in clinical applications.
Traditional targeted methods like Western Blot can only detect a limited number of targets at a time. Our Global Histone Modification Quantification Analysis service utilizes high-throughput mass spectrometry to systematically and unbiasedly identify and quantify all common histone modifications in a sample without pre-selection, painting a complete "histone modification map" for you.
Our Service Offerings
With this service, you will receive a systematic and detailed data analysis report, delivered through the following aspects:
| Service Item | Specific Content & Deliverables |
| Comprehensive Modification Identification | Systematically identify over 200 histone modification entries in a single run, without bias. |
| Precise Site Localization | Accurately pinpoint the specific histone type and amino acid residue where modifications occur (e.g., H3K4me3, H3K27ac). |
| Diverse Modification Types | Simultaneously analyze various modification forms including Acetylation (Ac), Mono-methylation (me1), Di-methylation (me2), and Tri-methylation (me3). |
| Accurate Quantification Data | Provide relative quantification intensity for each modification, enabling comparative analysis across different sample groups. |
| In-depth Crosstalk Analysis | Uncover potential interactions and regulatory networks between different modification sites. |
Service Advantages
- High-Throughput & High Coverage: A single experiment enables genome-wide modification scanning, significantly enhancing research efficiency and the potential for novel discovery.
- Simultaneous Multi-Modification Analysis: Our specialized workflow allows for the concurrent profiling of multiple modification types and sites, ideal for studying complex crosstalk.
- High Sensitivity & Accuracy: Leveraging high-precision mass spectrometry platforms ensures accurate identification and quantification even for low-abundance modifications.
- Expert Bioinformatics Support: We deliver not just raw data, but deeply mined, biologically interpretable insights.
Service Workflow
Contact our technical support to discuss your experimental design, then prepare and ship your samples according to guidelines.
Upon receipt, samples undergo quality control. Qualified samples proceed through histone extraction, digestion, and peptide purification.
Data acquisition is performed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
Raw data is processed through database searching, modification identification, quantification, and bioinformatic interpretation.
You receive a comprehensive project report including all result figures, tables, and detailed analysis descriptions.
Sample Submission Guidelines
To ensure project success and high-quality data, please adhere to the following guidelines:
- Sample Quality: Sample integrity is paramount. Ensure samples are fresh or properly stored at -80°C, avoiding repeated freeze-thaw cycles.
- Submission Requirements: Please prepare sufficient sample quantities as specified in the table below. We recommend a minimum of 3 biological replicates per condition for reliable results.
Sample Type Minimum Amount Notes Tissue 100 mg Remove irrelevant connective tissue when possible. Cells 7 x 107 cells Harvest cell pellets by centrifugation and wash thoroughly with PBS. - Technical Consultation: For special sample types or complex study designs, we strongly recommend consulting with our technical team prior to submission for personalized planning.
Let our professional histone modification analysis platform be your key tool for exploring epigenetics. Contact us today to unlock the gene regulatory secrets hidden within your samples!
