Glutaroylation Proteomics Service

Protein glutaroylation is a newly discovered and highly significant post-translational modification. This reversible process involves the covalent attachment of a glutaryl group (from donors like glutaryl-CoA) to lysine residues on substrate proteins, catalyzed by specific enzymes.

As a newly identified acylation modification, glutaroylation plays crucial regulatory roles in nucleosome assembly, chromatin structure, gene expression, DNA damage repair, and the cell cycle. Similar to other acylation modifications, it also plays a key role in metabolic processes. Research has shown that glutaroylation exerts specific effects on nucleosome dynamics and sperm motility.

Lysine residues normally carry a positive charge, but glutaroylation converts this to a negative charge. This alteration in protein charge can significantly impact protein conformation and protein-protein interactions. With rapid advances in proteomics and mass spectrometry technologies, glutaroylation is increasingly demonstrating its research value as an important member of the histone lysine acylation modification family.

Our Services

We provide comprehensive and professional glutaroylation proteomics analysis services, including:

• Glutaroylation Site Identification: Systematically identifies glutaroylated proteins and their precise modification sites using specific antibody enrichment and high-resolution mass spectrometry.
• Quantitative Glutaroylation Proteomics Analysis: Employs advanced quantitative strategies to accurately measure glutaroylation levels under different physiological or pathological conditions, revealing dynamic changes in modification patterns.
• Pan-Cellular Protein Glutaroylation Profiling: Utilizes optimized proteomics technologies to comprehensively analyze all glutaroylated proteins within cells, constructing a complete glutaroylation landscape.
• Functional Studies of Glutaroylation: Integrates bioinformatics analysis to deeply elucidate the biological functions of glutaroylation, including its effects on metabolic pathways, enzyme activity regulation, and cellular stress responses.

Service Advantages

• High-Specificity Enrichment: Uses optimized antibody affinity enrichment methods with high affinity and specificity for glutaroylated peptides, ensuring efficient recovery of low-abundance peptides from complex samples.
• Advanced Analytical Platform: Equipped with high-resolution, high-sensitivity mass spectrometry systems, guaranteeing accuracy and reliability in large-scale glutaroylated peptide identification.
• Comprehensive Coverage: Through optimized peptide separation and enrichment strategies, combined with necessary digestion and fractionation methods, significantly increases the number and coverage of identified glutaroylated proteins.
• Professional Bioinformatics Analysis: Provides a complete bioinformatics analysis pipeline, including modified protein and site identification, functional classification, metabolic pathway annotation, and protein-protein interaction network analysis.

Workflow

Sample Submission Guidelines

To ensure accurate experimental results, please refer to the following sample requirements:

All samples must be stored in liquid nitrogen or at -80°C and shipped with sufficient dry ice to avoid repeated freeze-thaw cycles.

Glutaroylation proteomics provides a powerful tool for deciphering the precise regulation of life processes. We look forward to supporting your research with professional and reliable technical services, exploring the mysteries of protein modifications together.