Post-Translational Modification PRM Targeted Validation
Parallel Reaction Monitoring (PRM) is a targeted protein quantification technology based on high-resolution, high-accuracy mass spectrometry, representing a significant upgrade from traditional SRM/MRM mass spectrometry quantification techniques. By selectively detecting target proteins or specific peptides (including those with post-translational modifications), this technology enables precise quantitative analysis of target molecules. With the rapid development of mass spectrometry technology, PRM has become a key tool for validating omics results in post-translational modification research, demonstrating significant value in biomarker validation, disease mechanism research, and drug development.
Figure 1. Schematic representation of parallel reaction monitoring (PRM) mass spectrometry. (Source: Bezstarosti Karel, et al. 2024)
The core advantage of PRM technology lies in its ability to utilize secondary fragment ions for relative or absolute protein quantification. Based on high-precision mass analyzers, PRM can specifically select precursor ions with predefined mass-to-charge ratios for fragmentation, then detect all fragment information generated by the peptide, achieving precise quantification through ion intensity calculations.
Our Services
We provide comprehensive and professional PRM targeted validation services for post-translational modifications, with specific service items as follows:
| Service Item | Service Description | Application Features |
| Phosphorylation Modification PRM Validation | Precise localization and quantitative analysis of protein phosphorylation sites | Revealing regulatory mechanisms in signal transduction pathways |
| Acetylation Modification PRM Validation | Detection of protein acetylation levels and analysis of its role in gene expression regulation | Exploring epigenetic regulatory mechanisms |
| Ubiquitination Modification PRM Validation | Analysis of protein ubiquitination status and study of protein degradation regulatory mechanisms | Analyzing protein stability regulation |
| Customized Modification Validation | Providing PRM validation services for other types of post-translational modifications based on client needs | Meeting personalized research requirements |
| Absolute Quantitative Analysis | Achieving absolute quantification of target proteins/modifications using stable isotope-labeled peptides as internal standards | Providing precise quantitative data |
Service Advantages
Our PRM targeted validation service is built on an advanced high-resolution mass spectrometry platform, enabling accurate identification and precise quantification of target modified peptides through specific ion selection and multiple fragment ion monitoring technology. This technology offers exceptional selectivity and sensitivity, effectively distinguishing co-eluting interference in complex samples and accurately detecting low-abundance modification signals. The quantification strategy based on secondary fragment ions ensures excellent data reproducibility and quantitative accuracy, while enabling high-throughput validation analysis of various post-translational modifications without relying on specific antibodies.
Workflow
Quality assessment upon sample receipt to ensure compliance with experimental requirements
Protein extraction using optimized protocols and digestion into peptides with specific proteases
Separation of peptide mixtures using nano-liquid chromatography to reduce sample complexity
PRM data acquisition based on high-resolution, high-mass-accuracy mass spectrometry platforms
Processing raw data using professional software, including chromatographic peak extraction, peak integration, and quantification
Ensuring result reliability through internal standard correction and data normalization
Providing complete reports including experimental procedures, instrument parameters, quantitative results, and bioinformatics analysis
Sample Submission Guidelines
To ensure accurate experimental results, please refer to the following sample requirements:
| Sample Type | Minimum Amount Required | Storage Conditions |
| Animal Tissue | ≥100 mg | Liquid nitrogen or -80°C |
| Plant Tissue | ≥500 mg | Liquid nitrogen or -80°C |
| Cell Samples | ≥2×10⁷ cells | Cell pellet preservation |
| Blood and Other Body Fluids | ≥1 ml | -80°C |
| Microorganisms | ≥300 mg | Liquid nitrogen or -80°C |
All samples should be protected from repeated freeze-thaw cycles and shipped with adequate dry ice to ensure sample quality.
PRM targeted validation technology provides a powerful and precise tool for post-translational modification research. We look forward to offering professional and reliable technical support for your research and jointly advancing the development of protein modification studies.
Reference
- Bezstarosti Karel, et al. Parallel reaction monitoring targeted mass spectrometry as a fast and sensitive alternative to antibody-based protein detection. Frontiers in Analytical Science. 2024;4.
