Redox Proteomics Services
Redox proteomics is an important branch of proteomics that focuses on studying reversible redox modifications of protein cysteine residues. These modifications play a central role in cellular signal transduction, metabolic regulation, and disease mechanisms. With growing research on oxidative stress-related diseases (such as cancer, neurodegenerative disorders, and metabolic diseases), redox proteomics has become a hotspot in the biomedical field, providing critical support for drug target discovery and biomarker development.
Cysteine residues in proteins are rich in nucleophilic sulfur atoms, making them highly reactive amino acids that not only participate in enzymatic catalysis but are also prone to extensive post-translational modifications. These modifications are primarily mediated by reactive oxygen species (ROS) and include disulfide bond (S-S) formation, S-glutathionylation (SSG), S-nitrosylation (SNO), S-sulfenylation (SOH), and non-redox modifications such as S-acylation (e.g., palmitoylation). These modifications can be reversibly reduced to free thiol groups (-SH) by reducing agents (e.g., glutathione), dynamically regulating protein conformation, activity, and function.
Redox modifications influence various cellular processes, including gene transcription, energy metabolism, signal transduction, apoptosis, and protein subcellular localization and transport. This reversible regulatory mechanism is crucial for maintaining cellular redox homeostasis, and its dysregulation is closely associated with numerous diseases, such as inflammation, cardiovascular diseases, and age-related pathologies. High-throughput analysis of these modifications helps uncover disease mechanisms and advance precision medicine.
Our Services
We provide comprehensive redox proteomics analysis services, specializing in the qualitative and quantitative detection of cysteine redox modifications:
- Modification Type Detection: Covers major modification forms, including disulfide bonds, S-glutathionylation, S-nitrosylation, S-sulfenylation, and S-acylation (including palmitoylation).
- High-Throughput Mass Spectrometry Analysis: Utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology for precise identification and quantification of modification sites in complex samples (e.g., cells, tissues, or body fluids).
- Bioinformatics Analysis: Includes modification site mapping, functional annotation (e.g., GO and KEGG pathway analysis), and interpretation of dynamic changes in modifications and their association with diseases.
- Customized Services: Offers targeted validation, time-series analysis, or multi-sample comparison plans based on client needs.
Service Advantages
We provide highly sensitive and accurate redox proteomics analysis services, employing optimized sample processing workflows and advanced mass spectrometry technologies to ensure reliable detection of low-abundance modifications. Our services cover multiple cysteine modification types, enabling comprehensive analysis of modification dynamics and functional correlations. Supported by a team of experienced biochemistry and bioinformatics experts, we offer end-to-end assistance from experimental design to functional interpretation. With standardized processes, we ensure fast turnaround times and rigorous quality control, significantly enhancing data reproducibility and research efficiency.
Service Workflow
Clients contact us via email or phone, providing sample information and research objectives. We assess requirements and customize the analysis plan.
Clients prepare samples (e.g., cell lysates or tissue extracts) according to guidelines and complete the sample information form. We provide detailed instructions for sample collection and preservation.
Upon sample receipt, we perform protein extraction, reducing agent treatment, enzymatic digestion, and mass spectrometry detection to ensure modification state stability.
Bioinformatics tools are used for modification site identification, quantitative analysis, and functional enrichment.
A detailed report is provided, including raw data, modification spectra, statistical results, and biological interpretation, supporting follow-up experimental design. Clients can track progress through our online system and access technical consultation throughout the process.
Important Notes
- Sample Requirements: Fresh or -80°C preserved samples are recommended, with avoidance of repeated freeze-thaw cycles. The sample amount must meet the minimum protein quantity requirement (typically ≥100 μg). Specific requirements will be detailed in the project plan.
- Result Interpretation: Redox modifications are susceptible to sample handling and environmental factors. We recommend incorporating biological replicates and control experiments to enhance reliability.
- Application Scope: This service is for research purposes only and not for clinical diagnosis. For special requirements (e.g., disease model analysis), please communicate with us in advance. For any questions, please contact our technical support team for further guidance.
Redox proteomics offers a unique perspective for understanding cellular signal transduction and disease mechanisms. Our professional service team, equipped with advanced technology platforms and extensive project experience, is committed to providing precise and reliable redox modification analysis solutions. We aim to support breakthroughs in disease mechanism research, drug target discovery, and biomarker development. Contact us to collaborate on advancing redox biology research.
