Exosome Proteomics Service
Exosomes are nano-sized vesicles secreted by cells that transport proteins, lipids, and nucleic acids to mediate intercellular communication and regulate processes such as immunity, metabolism, and the tumor microenvironment. Because exosome proteins closely mirror their parent cells' status, comprehensive proteomic analysis can reveal disease biomarkers, map signaling pathways, assess therapeutic response, and decode cellular communication networks—providing critical insights for early diagnosis, targeted therapy, and personalized medicine. Leveraging an advanced mass spectrometry platform and DIA strategies, Creative Diagnostics delivers deep, quantitative, and high-coverage profiling of exosome proteomes to help you uncover essential biological information.
Service Offerings
- Exosome isolation and quality assessment
- Direct-DIA, GPF-DIA, and staged DDA-guided DIA workflows
- Whole-proteome quantification and multi-statistical analyses
- Differential protein screening with volcano plots and heat maps
- Functional enrichment and pathway analysis (GO/KEGG/Reactome)
- Construction and interpretation of protein–protein interaction networks
- Customized biological insights and experimental design guidance
Key Advantages
Our DIA-based workflow delivers exceptional proteome coverage and sensitivity, overcoming the low-abundance limitations of traditional DDA methods. The integration of GPF and staged DDA-guided acquisition enhances quantitation accuracy and reproducibility. By combining global clustering, differential screening, and functional enrichment, we offer insights from overall proteome patterns down to individual protein behaviors. End-to-end service—from sample prep through final report—saves time and resources, while our expert bioinformatics team provides tailored interpretation and experimental recommendations to accelerate your research.
Workflow
Isolate and purify exosomes, quantify yield, and verify purity to ensure consistent input for mass spectrometry.
Tailor the optimal DIA approach—Direct-DIA, GPF-DIA, or staged DDA-guided DIA—based on your sample type and research goals.
Perform high-resolution DIA runs to capture the full exosome proteome with precise quantitation.
Use specialized software to deconvolute spectra, match peptides, and calculate protein abundances accurately.
- Global Profiling: cluster heat maps, Pearson correlation, PCA, and PLS-DA
- Differential Protein Selection: statistical summaries, volcano plots, comparative heat maps, and individual protein comparisons
- Functional Annotation: GO, KEGG and Reactome pathway enrichment, and protein–protein interaction networks
Provide clear data visualizations and comprehensive reports, including biological interpretation and recommendations for follow-up experiments.
Typical Applications
- Discovery and validation of disease biomarkers
- Studies of exosome-mediated cell–cell communication
- Drug target identification and mechanism-of-action research
- Development of diagnostic reagents and therapeutic targets
For more information or a customized proposal, please contact our technical team. We are committed to delivering professional, high-efficiency exosome proteomics solutions.
