Custom Enzyme Assay Services
Custom enzyme assays deliver accurate, quantitative evaluation of enzyme activity, kinetics, and regulation by selecting the ideal detection technology and reaction conditions for your target. Integrating luminescence, absorbance and immunoassays with automated execution and stringent quality controls, Creative Diagnostics offers end-to-end method development and data analysis to accelerate drug screening, enzyme engineering, and fundamental research.
Service Offerings
We leverage diverse detection platforms and automated workflows to provide precise, high-throughput custom enzyme assays:
- Luminescence Assays
ADP-Glo and ATP bioluminescence, plus chemiluminescent detection using HRP or AP. High sensitivity and low background make these assays ideal for detecting low-level enzyme activities and high-throughput screening. - Absorbance Assays
Classic substrate-product colorimetric measurements on multi-channel plate readers for real-time kinetic monitoring. Robust and easy to implement, suitable for initial activity screens and kinetic parameter determination. - Cell-Based Assays
- Kinetic Parameter Determination
Key Advantages
Backed by years of experience and our state-of-the-art automated platforms, we combine luminescence, absorbance and cell-based techniques to craft the perfect assay for your enzyme. Rigorous quality controls and robotic execution guarantee highly reliable, reproducible data. By fine-tuning substrates, inhibitors, and assay conditions, we deliver comprehensive kinetic analyses in minimal time, helping you pinpoint enzyme activity and mechanism to drive your research and development forward.
Applications
- Drug discovery and lead compound evaluation
- Enzyme engineering and mutant characterization
- Signaling pathway and regulatory mechanism studies
- Biosensor development and diagnostic reagent design
- Fundamental biochemical and cell biology research
Considerations
- Provide enzyme source or cell line details and sequence information
- Sign an NDA for proprietary or patent-sensitive molecules
- Ensure substrate and compound stability for reliable results
- Communicate special requirements (temperature, pH extremes) early for protocol adjustments
Partner with us for expertly developed, automated enzyme assays and kinetic studies—delivering reliable data to accelerate your scientific and product development goals.
