Protein Palmitoylation Proteomics Service
Palmitoylation modification, commonly referred to as S-palmitoylation, is a crucial post-translational lipid modification of proteins. It involves the covalent attachment of palmitic acid (a 16-carbon saturated fatty acid) to the sulfhydryl group of a cysteine residue in a protein via a thioester bond. Unlike other lipid modifications, S-palmitoylation is characterized by its unique reversibility. This "dynamic switch" property allows cells to precisely regulate protein membrane association, stability, subcellular localization, and protein interactions in both time and space, thereby influencing critical biological processes such as signal transduction, cell growth, and development. Research indicates that palmitoylation modification affects approximately 20% of the proteome, making it the most prevalent type of lipid modification.
Figure 1. Dynamic protein S-palmitoylation. (Source: Sobocińska J, et al. 2018)
Our Services
We offer professional and comprehensive protein palmitoylation proteomic detection and analysis services to help you accurately identify palmitoylated proteins and their specific sites in your samples.
| Service Item | Specific Content & Deliverables |
| Modification Site Identification | Utilizing efficient enrichment strategies and high-resolution mass spectrometry to systematically identify palmitoylated proteins and their specific modification sites in your samples, providing detailed information on modified peptides. |
| Differential Modification Analysis | Comparing modification profiles across different treatment groups (e.g., disease vs. healthy, drug-treated vs. control) to perform qualitative and relative quantitative analysis, screening for significantly altered palmitoylated proteins and sites to pinpoint key regulatory targets. |
| Customized Bioinformatics Analysis | In-depth data mining of identified modified proteins, including GO functional annotation, KEGG/Reactome pathway enrichment analysis, protein-protein interaction network construction, and more, to reveal the underlying biological significance. |
Service Advantages
- High-Specificity Enrichment: Advanced enrichment techniques effectively reduce non-specific binding, ensuring highly accurate and reliable modification site identification.
- High-Sensitivity Detection: Leveraging advanced mass spectrometry platforms enables efficient identification even for low-abundance palmitoylated proteins.
- Complete Solution: We provide a one-stop service encompassing sample preparation, modification enrichment, mass spectrometry detection, and in-depth bioinformatics analysis, ensuring seamless integration from data generation to biological interpretation.
- Expert Project Support: Our team possesses extensive experience in proteomics projects, offering professional support throughout the entire cycle, from experimental design to result interpretation.
Service Workflow
We discuss your research objectives and sample details to tailor the most suitable service plan for you.
Prepare and ship your samples according to the "Sample Submission Guidelines" below.
Upon receipt, samples undergo quality control, protein extraction, digestion, and palmitoylated peptide enrichment.
Enriched peptides are analyzed by Liquid Chromatography-Tandem Mass Spectrometry.
Raw data is processed for database search, qualitative/quantitative analysis, and deep bioinformatics mining, culminating in a comprehensive technical report delivered to you.
Sample Submission Guidelines
To ensure experimental success, please prepare your samples according to the following list. These amounts represent the minimum required for a single detection, and we recommend preparing additional backup samples depending on the number of replicate analyses needed.
| Sample Type | Minimum Amount |
| Conventional Animal Tissues | ≥ 300 mg |
| Soft Plant Tissues (e.g., leaf, flower) | ≥ 300 mg |
| Bacterial Pellet | ≥ 500 mg |
| Fungal Pellet | ≥ 8 g |
| Cells | ≥ 8 × 107 cells |
| Serum/Plasma | ≥ 1 mL |
| Urine | ≥ 50 mL |
We are committed to providing you with accurate and reliable, one-stop palmitoylation proteomics solutions. From rigorous sample analysis to in-depth data interpretation, we offer robust technical support throughout your research journey. Let's collaborate to explore the uncharted territories of protein functional regulation and accelerate your scientific discoveries.
Reference
- Sobocińska J, et al. Protein Palmitoylation and Its Role in Bacterial and Viral Infections. Front Immunol. 2018 Jan 19;8:2003.
