Autophagy Screening Service
Autophagy is a critical intracellular vesicle-mediated process responsible for organelle turnover, protein degradation, and stress response. Dysregulated autophagy is implicated in neurodegenerative diseases, cancer, infections, and metabolic disorders. High-throughput screening of autophagy pathways accelerates drug discovery and mechanistic research.
Service Offerings
1. GFP-LC3 WT/MT Screening System
- Wild-type (WT) and mutant (MT) LC3 fused to GFP for real-time monitoring of autophagosome formation.
2. GFP-LC3-RFP-LC3 MT Dual-Fluorescence System
- Dual labeling with GFP and RFP to distinguish autophagosome precursors from autolysosomes and quantify autophagic flux.
3. Luciferase-LC3 WT/MT Reporter System
- WT and MT LC3 fused to luciferase, with luminescence intensity reflecting autophagy levels; high sensitivity and low background.
Key Advantages
Our multi-mode detection platform integrates fluorescence imaging and luminescence assays to monitor autophagy initiation, flux, and completion. The system supports high-throughput workflows with built-in standard curves and stringent quality controls, ensuring excellent signal linearity and reproducibility. A collaborative team of cell engineering, imaging analysis, and data science experts manages every step, and we offer flexible customization of vector design, cell lines, and treatment conditions to meet specific project needs.
Workflow
Define research objectives, targets, and assay type to design a tailored screening plan.
Select and construct the optimal reporter vector based on client needs; verify expression efficiency and functionality via Western blot and fluorescence imaging.
Fine-tune cell seeding density, compound concentrations, and incubation times to establish ideal assay conditions.
Perform compound or genetic perturbations on automated platforms and capture signals.
Process raw data with background subtraction, normalization, and statistical analysis; generate comprehensive reports.
Deliver charts, hit lists, and recommendations for follow-up validation.
Typical Applications
- Screening of small molecules or natural products
- Assessment of gene knockdown/knockout effects on autophagy
- Investigation of autophagy-related protein interactions and mechanisms
- Dynamic monitoring of autophagic flux in disease models
- Optimization of lead compounds targeting autophagy regulators
Considerations
- Cell Line Selection: Autophagy levels vary across cell types; choose the most relevant model for your study.
- Fluorescence Interference: In dual-fluorescence assays, minimize spectral overlap by optimizing filter sets.
- Biosafety: All procedures comply with Biosafety Level 2 regulations to protect personnel and the environment.
For more information or to discuss a customized screening solution, please contact our technical support team.
