Targeted Protein Depletion Assay Services
Protein Depletion Assay is fundamentally reshaping the landscape of modern drug discovery. Unlike traditional "occupancy-driven" small molecule inhibitors, target protein depletion molecules utilize an "event-driven" mechanism. They hijack the cell's internal ubiquitin-proteasome system to completely degrade and clear disease-causing proteins, opening new doors for traditionally "undruggable" targets.
Accurate, sensitive, and reproducible target protein depletion assays are essential for evaluating drug efficacy, guiding structural optimization, and advancing your pipeline. Creative Diagnostics offers a one-stop bioassay platform designed to reveal the true activity and kinetics of your degraders within a realistic cellular microenvironment.
Our Services
Backed by extensive hands-on experience in the TPD field, our technical team provides customized support throughout your entire preclinical R&D cycle. To fully profile your degrader molecules, we utilize a multi-dimensional technology matrix covering everything from biochemical interactions to cellular phenotypes:
- Dynamic Live-Cell Tracking (Split Luciferase Assay): Moving beyond the limits of endpoint assays, we monitor ternary complex formation kinetics and target degradation pathways in real-time within a physiological live-cell environment.
- Homogeneous High-Throughput Screening: Dramatically boosting screening efficiency for large compound libraries.
- Classic Target Quantification (ELISA & Western Blot): Using industry "gold standards" for robust qualitative and absolute quantitative analysis of degradation levels, providing you with solid cross-validation data.
- Global Specificity Profiling (Mass Spectrometry Proteomics): Going beyond single-target limits to map the degradation profile across the entire proteome. We screen for off-target effects to ensure the safety and selectivity of your lead compounds.
- Downstream Cascade Assessment (qPCR): Tracking the broader biological impact of target degradation on core intracellular signaling pathways at the transcriptional level.
Our Advantages
- End-to-End Support: From early mechanism exploration to large-scale screening, our experts are involved at every step, providing seamless support from experimental design to final data delivery.
- Rigorous Cross-Validation: We combine biophysics, immunology, and proteomics to eliminate the false positives that can occur with single-platform testing, ensuring highly reliable data.
- Overcoming Technical Bottlenecks: Leveraging our practical experience, we deliver mature, custom solutions for common hurdles like insoluble degraders, low-abundance targets, and complex cell models.
Workflow
We analyze your target characteristics and degrader type to customize a cost-effective and scientifically rigorous assay strategy.
We build cell models specific to your project and determine the optimal reagent ratios, incubation times, and detection windows.
We perform standardized assays at your requested scale, ensuring data stability and reproducibility both within and across batches.
We deliver a comprehensive report featuring detailed methodologies, degradation kinetics curves, raw data, and expert conclusions.
Applications
- Lead compound discovery and structural optimization.
- Target selectivity evaluation and proteome-wide safety analysis.
- Ternary complex recruitment efficiency and ubiquitination kinetics studies.
- Verification of cellular phenotypes and Mechanisms of Action (MoA) mediated by target clearance.
