Antibody Humanization Service
Animal-derived antibodies (e.g., mouse antibodies) often trigger strong immune reactions in humans, leading to human anti-mouse antibody (HAMA) responses that compromise safety and efficacy. Our humanization technology retains the original CDRs while introducing "back mutations" in framework regions to maximize human sequence content. This molecular "disguise" reduces immune recognition and prevents HAMA responses, ensuring stable and safe clinical performance. The resulting humanized antibodies maintain the parent's affinity and specificity, offering reliable tools for drug development and clinical research. With our specialized platform, Creative Diagnostics can convert candidates from various sources into human-compatible molecules without sacrificing functionality, providing a solid foundation for therapeutic development.
Service Offering
We provide end-to-end humanization services for antibodies from multiple sources—mouse, camelid (camel/llama), rabbit, and chicken—including design, gene synthesis, expression validation, library construction, high-throughput screening, mammalian expression and purification, quantitative affinity analysis, and comprehensive reporting.
Key Advantages
- High Affinity Retention: CDRs remain intact, ensuring humanized antibodies match the parent's binding performance and specificity.
- Minimal Mutations: Only essential framework residues are back-mutated to reduce structural risk and improve success rates.
- Rapid Screening: High-throughput expression and grading deliver multiple high-affinity leads within 2–4 weeks.
- Low Immunogenicity: Maximized human sequence content significantly lowers HAMA risk and enhances clinical safety.
- Multi-Source Compatibility: Supports antibodies originating from mouse, camelid, rabbit, and chicken to meet diverse research needs.
- One-Stop Solution: From consultation and design through validation and reporting, we manage the entire process for a seamless experience.
Workflow
Thoroughly assess project background, target details, and application goals to define humanization objectives and success criteria.
Develop a CDR grafting and back-mutation plan based on the parent antibody's sequence and structure.
Synthesize or clone original mouse, camelid (camel/llama), rabbit, or chicken antibody genes and confirm expression and function.
Build a focused library of humanized variants with targeted framework mutations to streamline screening.
Express humanized candidates, rank them by binding strength, and select the top 3–5 clones.
Produce selected antibodies in mammalian cells and purify to high purity via affinity chromatography.
Measure association and dissociation rates of final candidates to quantify binding strength and confirm performance.
Deliver antibody sequences, purity metrics, affinity data, and process improvement suggestions to support your next steps.
Typical Applications
- Monoclonal antibody drug development
- Target optimization for antibody–drug conjugates (ADCs)
- Immunotherapy and cell therapy research
- Diagnostic reagent and imaging probe development
- Structural biology and epitope mapping
For more information or a tailored proposal, please contact our technical team. We are committed to delivering professional, efficient humanization solutions for your antibody projects.
