Targeted Lipidomics Solutions
Targeted lipidomics is a crucial branch of metabolomics focused on the precise identification and absolute quantification of specific lipid molecules in biological samples. Lipids are not only the primary components of cell membranes but also play key roles in energy storage, signal transduction, and cellular regulation. Through targeted analytical strategies, targeted lipidomics can accurately reveal changes in specific lipid metabolic pathways, providing reliable data support for research into metabolic mechanisms, biomarker discovery, and disease pathology.
Figure 1. Untargeted and targeted lipidomics methods in relation to the number of detected lipids and reliability of quantitative results. (Source: Munjoma N, et al. 2022)
Our Services
Creative Diagnostics provides high-sensitivity, high-specificity absolute quantification services for targeted lipids using LC-MS/MS coupled with Multiple Reaction Monitoring (MRM) technology. Our service enables precise detection of multiple lipid classes including fatty acids, glycerolipids, glycerophospholipids, sphingolipids, and glycolipids. We offer an end-to-end data solution encompassing lipid identification, absolute quantification, differential analysis, and pathway interpretation.
Service Advantages
- High Sensitivity and Accuracy: The MRM technique significantly enhances detection sensitivity and specificity, particularly suitable for the accurate quantification of low-abundance lipids.
- High-Throughput Analysis: Our optimized methodology allows for rapid processing of large sample batches, saving valuable time.
- Rigorous Quality Control: A comprehensive QC system is implemented throughout the entire process, from sample preparation to data analysis, ensuring experimental reproducibility and data reliability.
- Expert Analytical Team: Our experienced technical staff handles experimentation and data analysis, providing tailored bioinformatics interpretation and support.
- Customizable Solutions: We can customize or expand the lipid detection panel and analysis plan based on your specific research needs.
Workflow
We discuss your research objectives to determine the target lipid panel and sample requirements.
Standardized protocols are used for sample preprocessing and lipid extraction to ensure efficiency and consistency.
Instrumental data acquisition is performed.
Ion peaks are extracted for lipid identification and absolute quantification.
Rigorous QC is applied to quantitative data, followed by differential expression analysis, cluster analysis, pathway analysis, etc.
We deliver a comprehensive experimental report with all data files and provide necessary technical interpretation support.
Application
- Disease Mechanism Research: Investigating lipid metabolism dysregulation in diseases such as cardiovascular disease, neurodegenerative disorders, and cancer.
- Biomarker Discovery: Screening for potential lipid biomarkers related to disease diagnosis, prognosis, or therapeutic efficacy evaluation.
- Drug Development & Pharmacodynamics: Assessing the impact of drugs on lipid metabolic networks and exploring mechanisms of action.
- Nutrition & Food Science: Studying the regulatory effects of diet and nutritional components on lipid metabolism.
- Plant & Microbe Research: Analyzing lipid metabolic changes in processes like plant stress response and microbial synthesis.
Reference
- Munjoma N, et al. High Throughput LC-MS Platform for Large Scale Screening of Bioactive Polar Lipids in Human Plasma and Serum. J Proteome Res. 2022 Nov 4;21(11):2596-2608.
