SUMOylation Proteomics Analysis
SUMO (Small Ubiquitin-like Modifier) proteins are a class of small molecular proteins widely present in eukaryotic cells. SUMOylation is a reversible post-translational modification process that involves a series of enzymatic reactions, connecting mature SUMO molecules to specific lysine residues of target proteins via covalent bonds. While structurally similar to ubiquitination, SUMOylation differs functionally—it does not target proteins for degradation but precisely regulates protein function, stability, subcellular localization, and interactions. This modification process participates in regulating various critical cellular activities, including nucleocytoplasmic transport, transcriptional regulation, DNA damage response, cell cycle progression, and stress response. Abnormal SUMOylation is closely associated with multiple major diseases, such as cancer, neurodegenerative disorders, heart failure, and metabolic diseases, making it a current hotspot in biomedical research.
Our Services
| Service Category | Specific Analysis Items |
| SUMOylation Panoramic Analysis |
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| Molecular Mechanism Investigation |
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Service Advantages
We provide comprehensive, multi-level SUMOylation research solutions, employing multi-strategy combined analysis approaches that integrate various peptide enrichment methods and detection technologies to ensure complete coverage and high reliability in identifying SUMOylation sites. Based on high-resolution mass spectrometry platforms, our services achieve SUMOylation site mapping at single-amino acid resolution, providing site-specific modification information. Our technical solutions are applicable to various sample types, including animal tissues, cell samples, adipocytes, and related metabolic research models. Our professional team possesses extensive experience in SUMOylation modification research, offering one-stop services from experimental design, sample processing, mass spectrometry analysis to data interpretation, ensuring clients obtain reliable and interpretable research results.
Workflow
After receiving client samples, we conduct quality assessment to ensure they meet analytical requirements, including protein extraction, digestion, and peptide quantification quality control.
Selection of appropriate enrichment strategies based on research objectives, using methods such as antibody enrichment, combined peptide enrichment strategies, or dual affinity purification.
Utilizing nanoLC-MS/MS nanoscale chromatography for peptide separation, employing high-resolution mass spectrometers for data acquisition to obtain peptide sequence and modification site information.
Identifying SUMOylated proteins and sites through database searches, conducting quantitative comparisons and functional annotation analysis, and providing technical validation for key findings.
SUMOylation proteomics analysis service provides you with comprehensive and precise SUMOylation modification research solutions, supporting your scientific research to achieve breakthrough progress from basic mechanism studies to clinical application exploration. If you have any special requirements or questions, our professional technical team will provide detailed consultation and customized services.
