CAR-T Lead Antibody Discovery Service

Lead antibody identification is the cornerstone of CAR-T cell therapy development, directly impacting target recognition, intracellular signaling, and overall safety. High-quality lead antibodies must not only exhibit strong affinity and specificity but also internalize efficiently upon binding to target cells, delivering sustained activation signals for CAR-T cells. Unlike traditional antibody discovery, our integrated platform assesses both binding characteristics and internalization performance in parallel, ensuring selected candidates robustly target tumor antigens and demonstrate optimal uptake kinetics—ultimately enhancing the therapeutic potential and clinical success rate of CAR-T treatments.

Service Offerings

Creative Diagnostics delivers comprehensive, fully customizable support for CAR-T lead antibody projects, covering every step from initial library construction to functional validation:

• Library Construction & Strategy Design: Tailor phage or recombinant libraries to your antigen, combining solid- and solution-phase panning for maximum candidate diversity.
• Clone Selection & Sequencing: Perform high-throughput sequencing on enriched pools to pinpoint hundreds of lead candidates, ranked by affinity and sequence diversity.
• Antibody Humanization & Engineering: Retain CDRs while introducing back-mutations and optional Fc modifications or binding-site optimizations to boost stability and effector functions.
• Internalization Assessment: Quantify cell-surface binding rates, uptake efficiency, and time-course dynamics using flow cytometry and live-cell imaging to inform CAR design.
• Expression & Purification: Produce candidates in CHO or other mammalian systems and purify (>95% purity) with affinity chromatography and multi-step workflows to meet process development needs.
• In Vitro Functional Validation: Evaluate cell activation, cytotoxicity, and signaling pathways, providing a comprehensive pre-CAR-T biological assessment.
• Data Analysis & Reporting: Integrate screening results, sequencing data, internalization profiles, and functional assays into a detailed technical report with actionable optimization recommendations.

Key Advantages

• Efficient Screening: Advanced libraries and automated enrichment systems identify high-quality leads from billions of variants with hit rates above 20%.
• Precision Engineering: Combined humanization and Fc-region optimization balance affinity, stability, and effector function for multidimensional candidate refinement.
• Quantitative Internalization: Coordinated uptake assays deliver exact binding and internalization kinetics, supplying critical parameters for CAR design.
• One-Stop Solution: End-to-end service—from screening and sequencing to humanization, expression, purification, and validation—reduces handoffs and maximizes R&D efficiency.

Typical Applications

• CAR-T cell construct development and preliminary validation
• Lead antibody screening for antibody-drug conjugates (ADCs)
• Targeted pulse therapies and bispecific antibody engineering
• Immune cell internalization research and imaging probe development

For more details or a tailored proposal, please contact our technical team. We are committed to providing professional, high-efficiency lead antibody discovery solutions for your CAR-T projects.