Lentiviral vectors are third-generation self-inactivating gene delivery systems developed from HIV-1, belonging to the Retroviridae family. Compared to conventional gene transfer methods such as plasmid transfection and electroporation, lentiviruses offer superior transduction efficiency and broader host cell tropism. This technology has become a cornerstone tool in modern gene therapy, cell therapy, and genetic research, with extensive applications in CAR-T cell engineering, gene function studies, and genetic disease treatment.

Core Advantages of Lentiviral Packaging Technology

1. Broad Cell Infection Range

Unlike conventional retroviruses, lentiviruses efficiently infect both dividing and non-dividing cells. They can effectively transduce a wide range of mammalian cell types including neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, and hematopoietic stem cells. The infection efficiency is typically several to dozens of times higher than traditional plasmid transfection methods.

2. Efficient Foreign Gene Integration

Through reverse transcription, lentiviruses stably integrate foreign gene sequences into the host cell chromosome. Once integrated, these genes replicate with cell division, enabling long-term and stable expression of target genes. A single infection can produce persistent biological effects.

3. Adequate Gene Packaging Capacity

The system can carry relatively large foreign gene fragments with a maximum packaging capacity of 4.8 kb, sufficient for multi-gene co-expression, fusion protein tagging, and other complex genetic engineering applications.

4. Long-Term Stability of Gene Silencing

Lentiviral-based shRNA expression systems enable long-term, heritable transcriptional silencing of specific genes. After integration, shRNA sequences are processed by endogenous Dicer enzymes to generate siRNA, which binds to RISC complexes to continuously silence target genes. The silencing effect can be transmitted to daughter cells.

5. Superior Biosafety

6. Resistance to Gene Silencing

Service Offerings

We provide comprehensive lentiviral-based gene delivery solutions, including overexpression gene packaging, shRNA gene silencing design and packaging, and combined overexpression and gene interference strategies. Our services encompass the entire pipeline from vector optimization design, high-titer virus production, to comprehensive quality testing.

Application

• Gene Function Research
• CAR-T Cell Engineering
• Other Cell Engineering Therapies
• Genetic Disease Gene Therapy
• Stem Cell Engineering and Directed Differentiation
• Tumor Modeling and In Vitro Research

Considerations

1. Provision of Target Genes and shRNA

If customers provide their own target genes or shRNA sequences for packaging, the following information must be provided:

• Gene name (official abbreviation and full name)
• Species origin and NCBI accession numbers (GenBank ID or RefSeq ID)
• CDS coding sequence
• Expression plasmid map containing the gene sequence
• Relevant restriction enzyme cutting sites and cloning strategy information
• Gene optimization requirements (whether codon optimization or removal of internal promoters is needed)

2. Quality Standards and Delivery Specifications

Our standard delivery products include:

• Lentiviral expression plasmids containing target genes or shRNA sequences
• High-titer lentiviral solutions
• Negative control lentiviral solutions
• Complete experimental reports

3. Intellectual Property and Confidentiality

We commit to strict confidentiality of customer-provided gene information and our packaging process methods, and will not use this information for other commercial purposes.

If you have any questions about our lentiviral packaging services or need to develop a customized technical plan for your specific project, please feel free to contact us. Our technical team is ready to provide professional consultation and support.