Succinylation Proteomics Service
Protein succinylation is a recently discovered and highly significant post-translational modification process. It involves the transfer of a negatively charged four-carbon succinyl group to lysine residues on proteins, mediated by succinyl-CoA. This modification is widely present in both eukaryotic and prokaryotic cells and represents an evolutionarily conserved modification type.
Succinylation modification possesses unique biochemical characteristics: it not only introduces a two-unit charge change at the modified residue (similar to phosphorylation effects) but also adds a bulky group. These combined changes significantly influence protein structure and function. This modification dynamically regulates several key metabolic signaling pathways, including the tricarboxylic acid cycle, amino acid metabolism, and fatty acid metabolism.
Our Services
We provide comprehensive and professional succinylation proteomics analysis services, including:
- Succinylation Site Identification: Systematically identifies succinylated proteins and their precise modification sites using specific antibody enrichment and high-resolution mass spectrometry.
- Quantitative Succinylation Proteomics Analysis: Employs advanced quantitative strategies to accurately measure succinylation levels under different physiological or pathological conditions, revealing dynamic changes in modification patterns.
- Pan-Cellular Protein Succinylation Profiling: Utilizes optimized proteomics technologies to comprehensively analyze all succinylated proteins within cells, constructing a complete succinylation landscape.
- Functional Studies of Succinylation: Integrates bioinformatics analysis to deeply elucidate the biological functions of succinylation, including its effects on protein stability, subcellular localization, metabolic enzyme activity, and molecular interactions.
Service Advantages
- High-Specificity Enrichment: Uses optimized antibody affinity enrichment methods with high affinity and specificity for succinylated peptides, ensuring efficient recovery of low-abundance peptides from complex samples.
- Advanced Analytical Platform: Equipped with high-resolution, high-sensitivity mass spectrometry systems, guaranteeing accuracy and reliability in large-scale succinylated peptide identification.
- Comprehensive Coverage: Through optimized peptide separation and enrichment strategies, combined with necessary digestion and fractionation methods, significantly increases the number and coverage of identified succinylated proteins.
- Professional Bioinformatics Analysis: Provides a complete bioinformatics analysis pipeline, including modified protein and site identification, functional classification, metabolic pathway annotation, and protein-protein interaction network analysis.
Workflow
Protein extraction and quantification are performed using standardized protocols based on the sample type.
Protein samples are digested using specific proteases, with multi-enzyme strategies employed to increase coverage.
Succinylated peptides from the digest are enriched using succinylation-specific antibodies via immunoaffinity purification.
Enriched peptides are separated by high-performance liquid chromatography and analyzed by high-resolution mass spectrometry.
Raw MS data is acquired and processed using professional software for database searching and statistical analysis.
A comprehensive bioinformatics analysis is conducted on the identified succinylated proteins and sites to uncover their biological significance.
A detailed technical report is provided, including the experimental procedure, MS parameters, identification results, and bioinformatics analysis content.
Sample Submission Guidelines
To ensure accurate experimental results, please refer to the following sample requirements:
| Sample Type | Minimum Amount Required |
| Plant Leaves | Wet weight ≥ 0.8 g |
| Plant Roots, Stems, Xylem, Phloem, etc. | Wet weight ≥ 10 g |
| Cell Samples | Cell count equivalent to wet weight ≥ 8×10⁷ |
| Tissue Samples (Human, Animal, Microbial) | Wet weight ≥ 300 mg |
| Body Fluid Samples | Serum/Plasma volume ≥ 1 ml, CSF ≥ 1 ml, Urine ≥ 50 ml |
All samples must be stored in liquid nitrogen or at -80°C and shipped with sufficient dry ice to avoid repeated freeze-thaw cycles.
Succinylation proteomics provides a powerful tool for deciphering the precise regulation of life processes. We look forward to supporting your research with professional and reliable technical services, exploring the mysteries of protein modifications together.
