Ubiquitination Proteomics Analysis
Ubiquitination is a widely present and highly conserved post-translational modification process in eukaryotic cells. Through the coordinated action of a series of specific enzymes—including ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin ligases (E3)—ubiquitin molecules are covalently attached to target proteins, enabling specific modification of intracellular proteins. Ubiquitination plays a central role in the localization, metabolism, function, regulation, and degradation of proteins. It mediates the specific degradation of proteins via the ubiquitin-proteasome system and is extensively involved in the precise regulation of nearly all life processes, including cell cycle, proliferation, apoptosis, differentiation, metastasis, gene expression, transcriptional regulation, signal transduction, damage repair, and inflammatory immunity.
Recent research indicates that the precise regulation of the ubiquitin-proteasome system forms a stable and complex ubiquitination signaling network. Dysfunction of this network is closely associated with the development and progression of various major diseases, including cancer, neurodegenerative diseases, and metabolic disorders. This critical mechanism has become a key focus in biochemical research and offers promising targets for new drug development.
Our Services
We provide comprehensive and professional ubiquitination proteomics analysis services, including:
- Ubiquitination Site Identification: Utilizing high-specificity antibody enrichment methods combined with high-resolution mass spectrometry to systematically identify ubiquitinated proteins and their precise modification sites in samples.
- Quantitative Ubiquitination Proteomics Analysis: Employing various quantitative strategies (including label-based and label-free techniques) to accurately measure ubiquitination levels under different physiological or pathological conditions, revealing the dynamic changes in ubiquitination.
- Pan-Cellular Ubiquitination Profiling: Using advanced proteomics technologies to comprehensively analyze all ubiquitinated proteins within cells, constructing a complete ubiquitination landscape.
- Functional Studies of Ubiquitination: Integrating bioinformatics analysis to deeply elucidate the biological functions of ubiquitination, including its effects on protein stability, subcellular localization, activity, and molecular interactions.
Service Advantages
Our ubiquitination proteomics service platform offers the following core strengths:
- High Specificity Enrichment: Utilizes mainstream antibody affinity enrichment methods with high affinity for ubiquitinated peptides, ensuring efficient recovery of low-abundance ubiquitinated peptides from complex samples.
- Advanced Analytical Platform: Equipped with high-resolution, high-sensitivity mass spectrometry systems, guaranteeing accuracy and reliability in large-scale ubiquitinated peptide identification.
- Comprehensive Analysis Workflow: Employs peptide pre-fractionation and optimized enrichment strategies to significantly increase the number of identified ubiquitinated proteins and maximize the capture of ubiquitination information.
- Professional Bioinformatics Analysis: Provides a complete bioinformatics analysis pipeline, including modified protein and site identification, functional classification, pathway annotation, and protein-protein interaction network analysis.
Workflow
Protein extraction and quantification are performed using standardized protocols based on the sample type.
Protein samples are digested using proteases like Trypsin, with multi-enzyme strategies employed when necessary to increase coverage.
Ubiquitinated peptides from the digest are enriched using specific antibodies via immunoaffinity purification.
Enriched peptides are separated by nano-liquid chromatography and analyzed by high-resolution mass spectrometry.
Raw MS data is acquired and processed using professional software for database searching and statistical analysis.
A comprehensive bioinformatics analysis is conducted on the identified ubiquitinated proteins and sites to uncover their biological significance.
A detailed technical report is provided, including the experimental procedure, MS parameters, identification results, and bioinformatics analysis content.
Sample Submission Guidelines
To ensure accurate experimental results, please refer to the following sample requirements:
| Sample Type | Minimum Amount Required |
| Plant Leaves | Wet weight ≥ 0.8 g |
| Plant Roots, Stems, Xylem, Phloem, etc. | Wet weight ≥ 10 g |
| Cell Samples | Cell count equivalent to wet weight ≥ 8×10⁷ |
| Tissue Samples (Human, Animal, Microbial) | Wet weight ≥ 300 mg |
| Body Fluid Samples | Serum/Plasma volume ≥ 1 ml, CSF ≥ 1 ml, Urine ≥ 50 ml |
All samples should be stored in liquid nitrogen or at -80°C and shipped with an adequate amount of dry ice to avoid repeated freeze-thaw cycles.
Ubiquitination proteomics provides a powerful tool for deciphering the fine-tuned regulation of life processes. We look forward to offering you professional and reliable technical support for your research, exploring the mysteries of protein modifications together.
