Peptide Modification Services
Peptides—short chains of amino acids linked by peptide bonds—offer high biological activity and targeting precision. However, unmodified peptides often suffer rapid enzymatic degradation, short in vivo half-lives, and poor cell permeability. Through chemical or enzyme-catalyzed modifications, Creative Diagnostics introduces functional groups or structural motifs to:
- Enhance stability with terminal acylation/amidation or PEGylation to block nonspecific reactions and resist proteases
- Improve targeting by lipidation or cyclization to increase hydrophobicity and membrane affinity, or by biotinylation and chelator coupling for purification and imaging
- Load functionality via phosphorylation to mimic signaling or click chemistry to attach fluorescent, radioactive, or MRI probes for dynamic tracking
- Control conformation through head-to-tail or side-chain cyclization to lock active structures and boost binding affinity
Creative Diagnostics cover acylation/amidation, biotinylation, cyclization, alkylation, lipidation, PEGylation, phosphorylation, bifunctional chelator and imaging-probe coupling, and click-chemistry labeling. We continually expand our toolkit to meet cutting-edge research needs.
Service Offerings
Below is a concise overview of our core modification types and their applications:
| Modification Type | Goal | Methodology | Applications |
| Acylation/Amidation | Enhance stability; extend half-life | Chemical acylation and amidation | In vivopharmacokinetic studies |
| PEGylation | Reduce immunogenicity; increase solubility | PEG–NHS, PEG–maleimide reactions | Long-acting peptide therapeutics |
| Biotinylation | Facilitate purification and detection | Biotin–PEG reagents | Immunoprecipitation; affinity chromatography |
| Cyclization | Fix conformation; increase affinity | Head-to-tail or side-chain cyclization reagents | Receptor–ligand interaction studies |
| Alkylation/Lipidation | Improve membrane permeability | Long-chain alkyl or lipid reagents | Cellular targeting; in vivo delivery |
| Phosphorylation | Mimic signaling modifications | Chemical phosphorylation reagents | Signaling pathway and phosphorylation research |
| Chelator/Probe Coupling | Create imaging and diagnostic probes | DOTA/NODAGA reagents; fluorescent probes | PET/SPECT/MRI imaging; fluorescence imaging |
| Click Chemistry | Enable modular orthogonal labeling | Azide–alkyne cycloaddition (CuAAC) | Multimodal labeling and targeted delivery |
Key Benefits
With extensive expertise in peptide chemistry and state-of-the-art analytical platforms, we tailor and combine modification methods to precisely functionalize peptides at scale. Rigorous MS and HPLC quality control ensures consistency and reliability. Our agile approach lets us rapidly adjust strategies to your needs, accelerating your project timeline.
Workflow
peptide sequence, modification goals, and application context
implement the chosen chemical transformations
RP-HPLC, MS/NMR analysis
stability, permeability, and targeting assays
modified peptide and comprehensive analytical dossier
Considerations
- Provide accurate peptide sequence and modification requirements
- Some modifications may affect solubility; specify solvent preferences
- For in vivo or cellular use, share safety and biocompatibility criteria
- Custom scale or purity requirements may impact turnaround time and cost
Choose our peptide modification services to obtain high-stability, high-activity, and multifunctional peptides, empowering your research and product development. Contact us for tailored solutions and quotations.
