Protein Qualitative Analysis Service
Protein qualitative analysis is a core technology in proteomics research, aimed at comprehensively and accurately identifying the protein composition in biological samples. Creative Diagnostics utilizes the LC-MS/MS technology platform, combined with professional bioinformatics analysis, to achieve high-throughput identification of proteins in various samples such as cells, tissues, and body fluids, providing reliable data support for your scientific research.
Our protein qualitative analysis service involves digesting complex mixtures of in-solution protein molecules or SDS-PAGE bands into peptide mixtures. These are then separated by HPLC, analyzed by MS/MS, and finally identified through a professional analysis workflow, enabling the simultaneous identification of hundreds to thousands of proteins.
Service Offerings
- In-Solution Digestion Protein Identification: Suitable for protein solution samples, achieving comprehensive identification of soluble proteins through an efficient digestion process.
- In-Gel Digestion Protein Identification: Targeted analysis of protein bands or spots separated by SDS-PAGE, involving in-gel digestion and mass spectrometry analysis, particularly suitable for the precise identification of specific proteins.
- Comprehensive Profiling of Complex Mixture Samples: Full proteome identification of protein mixtures in complex samples like tissues, cells, and body fluids, capable of identifying hundreds to thousands of proteins in a single run.
- Customized Analysis for Special Samples: Providing tailored pre-processing and identification solutions for challenging samples such as FFPE tissues and membrane proteins.
Service Advantages
- Comprehensive Coverage & High Sensitivity
Our service enables large-scale protein identification, comprehensively covering a vast number of proteins in the sample—from a few to over a thousand proteins identified simultaneously. Leveraging advanced technology platforms and optimized pre-processing techniques, even low-abundance proteins can be effectively identified. - High Throughput & Standardization
We have established standardized, rapid, high-throughput proteomic analysis workflows, significantly enhancing research efficiency and suitability for large-scale sample projects. Standard projects are typically completed within specified working days. - Professional Analysis & Accurate Identification
Utilizing multi-dimensional data analysis strategies combined with professional bioinformatics analysis methods, we ensure the accuracy and reliability of identification results. Our professional technical team provides comprehensive data interpretation and analysis support.
Workflow
A dedicated technical consultant coordinates with you to clarify research objectives and sample characteristics.
Prepare and ship samples according to the provided sample preservation/shipping guidelines.
The entire process of sample processing, separation, and detection is completed within standardized laboratories.
Ensure data reliability through a multi-parameter quality assessment system.
- Protein identification list
- Basic protein information analysis
- Quality control report
- Delivery of raw data and analysis report
Applications
Our protein qualitative analysis service is widely used in:
- Identification of Unknown Proteins: Qualitative analysis of proteins with unknown functions.
- Interaction Protein Analysis: Identification of components in protein complexes obtained through techniques like Co-IP or Pull-down.
- Protein Modification Site Verification: Preliminary identification and verification of protein modification sites.
- Cell, Tissue, and Body Fluid Analysis: Comprehensive analysis of protein composition in various biological samples.
- Biomarker Screening: Providing fundamental data for disease biomarker research.
- Drug Target Identification: Assisting in drug mechanism of action studies.
Sample Submission Guidelines
To ensure reliable experimental results, please adhere to the following sample submission requirements:
| Sample Type | Sample Requirements |
| Protein Solution | Total protein amount >5 μg, concentration >0.1 μg/μl; Buffer should not contain detergents like NP-40, Triton X-100, etc. |
| SDS-PAGE Bands | Coomassie Brilliant Blue or silver stained (mass spectrometry compatible) bands must be clearly visible. |
| Cell Sample | Cell count ≥1×10⁷ cells. |
| Tissue Sample | Tissue amount ≥20 mg. |
Our protein qualitative analysis service is supported by an experienced technical team, managing the entire process from sample pre-processing to data interpretation. We ensure the provision of accurate, reliable, publication-ready experimental data, offering powerful technical support for your scientific research.
