Protein Phosphorylation Proteomics

Protein phosphorylation is one of the most crucial and widespread post-translational modifications, often regarded as the "molecular switches" that regulate cellular functions. By reversibly adding phosphate groups, it precisely controls protein activity and function, participating in nearly all vital biological processes, including cell proliferation, differentiation, signal transduction, apoptosis, and metabolism. In-depth analysis of the protein phosphorylation landscape is key to unraveling the mechanisms of life and the essence of disease. By systematically mapping phosphorylation profiles, we can identify critical biomarkers associated with disease initiation, progression, and therapeutic response, providing a fundamental basis for drug target discovery and novel diagnostic methods.

In recent years, high-throughput proteomics technologies based on Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) have matured. Combined with efficient phosphopeptide enrichment strategies, advanced mass spectrometry fragmentation techniques, and powerful bioinformatics tools, we can now perform systematic, precise mapping and quantitative analysis of tens of thousands of phosphorylation sites from complex samples in a single experiment, thereby fully revealing the hidden phosphorylation regulatory networks within biological samples.

Our Services

Creative Diagnostics provides a one-stop solution for phosphorylation proteomics analysis, including:

• High-Efficiency Phosphopeptide Enrichment: Utilizing advanced enrichment materials to ensure high specificity and recovery rates.
• LC-MS/MS Analysis: Employing high-resolution, high-mass-accuracy mass spectrometry platforms for data acquisition.
• Global Phosphorylation Site Identification & Quantification: Unbiased, in-depth profiling and relative quantification of phosphorylation sites in samples.
• Advanced Bioinformatics Analysis:
  • Phosphorylation site motif analysis
  • Screening of differentially phosphorylated proteins/sites
  • GO functional annotation and KEGG pathway enrichment analysis
  • Kinase-substrate interaction network prediction

Service Advantages

Our core competitive edge lies in the seamless integration of deep coverage, precise quantification, and professional support. Leveraging optimized enrichment protocols and high-precision mass spectrometry platforms, we enable deep mining of tens of thousands of phosphorylation sites in complex samples, delivering stable and reliable quantitative results. From initial project design to the final report, we provide professional technical support throughout the entire process, ensuring you obtain high-quality data and clear biological insights to efficiently achieve your research goals.

Service Workflow

Considerations

• Sample Integrity: Ensure samples are rapidly frozen after collection and avoid repeated freeze-thaw cycles to best preserve the native phosphorylation state.
• Protease/Phosphatase Inhibitors: Always include appropriate protease and phosphatase inhibitors during sample preparation to prevent protein degradation and loss of modifications.
• Avoid Contamination: Avoid introducing common detergents (e.g., SDS) or high concentrations of salts that can interfere with MS analysis.
• Biological Replicates: To ensure statistical power, we strongly recommend providing at least 3 biological replicates per group.

Protein phosphorylation proteomics is a powerful tool for deciphering fundamental biological mechanisms and disease progression. Leveraging our robust technology platform, rigorous quality control, and deep data analytics, we are committed to delivering a comprehensive and reliable phosphorylation research solution. Our service enables precise identification of key regulatory sites, accelerating both fundamental scientific discoveries and translational applications.