Folate Metabolism Detection
Folate (Vitamin B9) is an essential water-soluble vitamin crucial for sustaining life. It plays a central role in key physiological processes such as DNA synthesis and repair, amino acid metabolism, and the methylation cycle. Its metabolic status is directly linked to homocysteine levels, cell proliferation, neurodevelopment, and cardiovascular health. Targeted metabolomics technology, particularly platforms based on LC-MS/MS, enables precise and specific quantitative analysis of folate and its key metabolites within the body. This provides high-value data support for disease risk early warning, nutritional status assessment, medication guidance, and scientific research.
Our Services
Creative Diagnostics specializes in providing efficient and precise targeted detection and analysis services for folate metabolism. Utilizing an advanced LC-MS/MS platform, we offer accurate qualitative and absolute quantitative analysis of 7 core folate-related metabolites in various biological samples (such as serum, plasma, tissue, cells, etc.):
| Detected Metabolite | Primary Function/Significance |
| Folate | Assesses baseline nutritional intake levels. |
| 5-Methyltetrahydrofolate | The primary active form in circulation; a key indicator for assessing bioavailability. |
| 5-Formyltetrahydrofolate | Another active form, involved in specific biochemical reactions. |
| Homocysteine | A key intermediate in metabolism; elevated levels are a risk marker for cardiovascular, cerebrovascular, and neurological diseases. |
| S-Adenosylmethionine (SAM) | The primary methyl donor, involved in the methylation regulation of DNA, proteins, etc. |
| S-Adenosylhomocysteine (SAH) | A metabolite of SAM; the SAM/SAH ratio reflects the overall cellular methylation potential. |
| p-Aminobenzoylglutamate | One of the terminal metabolites of folate, aiding in understanding the overall metabolic pathway status. |
Service Advantages
Our service is built upon a high-precision LC-MS/MS platform, offering high-sensitivity and high-specificity detection capabilities. It enables the simultaneous and accurate quantification of seven key metabolites, providing a comprehensive analysis of the folate metabolic pathway. The process is high-throughput with a short turnaround time and is compatible with various biological sample types. We deliver in-depth reports featuring detailed data and expert biological interpretation, supported by strict quality control measures throughout the entire workflow—from sample receipt to data analysis—ensuring accuracy and reliability of results.
Workflow
The client states their detection needs. We assist in finalizing the testing protocol based on the research objectives and sample types.
The client prepares and ships samples according to requirements. Upon receipt, we assign unique identifiers and log sample information.
Samples undergo standardized preprocessing in a controlled laboratory environment, followed by targeted metabolite analysis using the LC-MS/MS platform.
Raw data is processed and quantitatively analyzed. A comprehensive written report is generated, containing detailed results, quality control charts, and preliminary interpretation.
The final report is delivered promptly. We provide necessary technical consultation and support for result interpretation.
Applications
- Clinical Research & Disease Risk Assessment: Used in studies related to cardiovascular diseases, neurological disorders (e.g., Alzheimer's disease), birth defects, cancer, etc., to explore the association between folate metabolism dysregulation and disease pathogenesis.
- Nutritional & Health Status Evaluation: Precisely assesses the folate nutritional status and metabolic capacity of individuals or specific populations, providing a basis for personalized dietary supplementation or clinical intervention.
- Drug Efficacy & Safety Monitoring: Monitors changes in metabolite levels following the administration of drugs like folate or methotrexate, aiding in efficacy evaluation or dosage adjustment guidance.
- Health Management & Precision Prevention: Serves as part of advanced health management programs, helping healthy or sub-healthy individuals understand their methylation metabolism status for early risk intervention.
- Life Science Research: Provides crucial targeted metabolomics data for fundamental research areas such as cell metabolism, animal model studies, plant physiology, and microbial metabolism.
