Precise Detection of Neutrophil and Monocyte Functional Subsets
Neutrophils and monocytes are the core forces of the body's innate immune system, acting as the "rapid response team" and the "versatile reserve force." They are not only the first responders to pathogen invasion, but changes in their quantity, proportion, and functional subsets also serve as a critical window for assessing the body's inflammatory status, infection type, immune balance, and certain disease progression.
While traditional complete blood counts only provide cell numbers, Creative Diagnostics utilizes advanced detection technologies to perform fine subtyping of neutrophil and monocyte functional subsets and calculate key indicators such as the Neutrophil-to-Lymphocyte Ratio (NLR). This provides you with deeper, more dynamically significant immunological data for mechanistic research, efficacy monitoring, and prognostic evaluation in fields like tumor immunology, infectious and inflammatory diseases, and autoimmune disorders.
Our Services
Creative Diagnostics offers the following precise detection items for human peripheral blood neutrophils and monocytes, revealing their complex functional states:
| Detection Item | Primary Significance and Clinical/Research Value |
| CD14- CD11b+ (Total Neutrophils) | Reflects the overall level of mature neutrophils in circulation, a core indicator of acute inflammatory response. |
| CD14- CD11b+ CD15+ (Chemotactic/Migratory Neutrophil Subset) | A subset with high CD15 expression, possessing enhanced ability for directional migration to inflammatory sites. Its changes indicate the intensity of inflammatory chemotaxis. |
| CD14- CD11b+ CD16+ (Killing/Activated Neutrophil Subset) | A subset with high CD16 expression, exhibiting stronger phagocytic and bactericidal activity. It is the main force for pathogen clearance, and its proportional changes correlate with infection control. |
| CD14+ (Total Monocytes) | Reflects the total monocyte pool, the precursors to macrophages and dendritic cells, linking innate and adaptive immunity. |
| CD14+ CD15+ (Chemotactic Monocyte Subset) | A subset with a specific chemotactic phenotype, associated with infiltration into specific tissues (e.g., tumors or atherosclerotic plaques). |
| CD14+ CD16+ (Intermediate/Inflammatory Monocyte Subset) | A subset with pro-inflammatory properties, capable of producing large amounts of inflammatory cytokines. It is often significantly elevated in chronic inflammation, sepsis, and certain autoimmune diseases. |
| Neutrophil-to-Lymphocyte Ratio (NLR) | An important marker of systemic inflammation and immune balance. An elevated NLR often indicates stronger systemic inflammatory response or immunosuppressive status, holding significant value in assessing tumor prognosis and risk of severe infection. |
Service Advantages
Our core strength lies in providing in-depth analytical services from sample to report, built upon a professional testing platform and stringent quality system. We go beyond precise cell counting by specializing in the functional subset analysis of neutrophils and monocytes (e.g., CD16+ killing subsets, CD16+ inflammatory monocytes) and providing key composite indicators like NLR. Combined with our one-stop solution capability, we can construct a comprehensive assessment profile spanning from innate to adaptive immunity for you. All processes adhere to standardized operations and quality control, ensuring data reliability. We deliver detailed reports efficiently with a standard turnaround time of approximately one week, fully supporting your scientific discoveries and clinical insights.
Workflow
After contacting us to confirm the testing plan, please collect peripheral blood using heparin sodium anticoagulation tubes and ship the samples under 2-8°C refrigeration.
The laboratory verifies sample information and completes quality control checks, then immediately proceeds with cell separation, fluorescence staining, and instrument-based detection.
The raw data undergoes professional analysis and review, culminating in the generation and delivery of a formal test report containing detailed results and interpretation.
Notes
To ensure smooth testing progress, please pay special attention to the following:
- Sample Requirements: Please use heparin sodium anticoagulation tubes (green cap) to collect fresh peripheral blood. Gently invert the tube to mix immediately after collection to avoid clotting or hemolysis. It is recommended that samples arrive at the laboratory within 24-48 hours of collection and be stored temporarily at 2-8°C.
- Special Processing: For projects requiring periodic sample collection, heparin sodium anticoagulated peripheral blood can be processed by isolating PBMCs, followed by cryopreservation in liquid nitrogen using serum with 10% DMSO. For serum samples, separate and store at -80°C, avoiding repeated freeze-thaw cycles.
- Deliverables: You will receive a formal electronic report containing the raw data, analytical charts, and brief interpretations for all detection items.
