Precise TMAO Quantification Service
Trimethylamine N-oxide (TMAO) is a gut microbiota-dependent metabolite that has garnered significant research interest. Its production begins in the gut, where specific bacteria produce trimethylamine (TMA) lyase. This enzyme converts dietary precursors such as choline, betaine, and carnitine into trimethylamine (TMA). Following absorption, TMA travels to the liver where it is ultimately oxidized to form TMAO by the enzyme flavin-containing monooxygenase (FMO). This process highlights the intricate interaction between the host and its symbiotic microbes.
Extensive research in recent years has established a strong link between TMAO and several major human diseases. It is recognized as an independent risk predictor for cardiovascular diseases and is associated with the progression of chronic kidney disease (CKD), renal insufficiency, and increased mortality risk. Furthermore, TMAO plays a significant role in the development and progression of type II diabetes, insulin resistance, non-alcoholic fatty liver disease, and certain cancers. Consequently, the accurate quantification of TMAO and related metabolites in biological samples has become a critical component of cutting-edge life science and clinical research.
Our Targeted Metabolomics Detection Service
Creative Diagnostics specializes in providing high-precision, high-throughput targeted metabolomics solutions. A core service we offer is the absolute quantification of key substances within the TMAO metabolic pathway. Our service extends beyond TMAO itself to cover the core substances in its complete metabolic pathway, providing clients with a more comprehensive biological perspective.
| Detected Substance | Role in the TMAO Pathway |
| Trimethylamine N-oxide | Terminal metabolite, core research target |
| Trimethylamine | Direct precursor of TMAO |
| Choline | Primary dietary precursor |
| Betaine | Primary dietary precursor |
| Carnitine | Primary dietary precursor |
| Creatinine | Kidney function marker for data correction |
Service Advantages
Our core service advantage lies in the use of the LC-MS/MS platform with MRM methodology, which allows for the absolute quantification of TMAO and its complete metabolic pathway substances with high specificity and sensitivity. The method is high-throughput and offers stable turnaround times, meeting the demand for precise detection of large sample batches. Furthermore, by combining professional analytical support with extensive project experience, we not only ensure data reliability but also provide deep support to transform your data into meaningful biological insights.
Workflow
Our specialist team will communicate with you to understand your specific research objectives, sample types, and quantities, working together to determine the optimal detection plan.
Please prepare your samples according to the "Sample Shipping Requirements" below and ship them promptly using sufficient dry ice (recommended standard: 5 kg per day of transit).
Upon receipt, samples are logged and aliquoted. They then undergo standardized processing including metabolite extraction, derivatization (if required), followed by LC-MS/MS analysis.
Raw data is acquired alongside rigorous quality control assessments. All quantitative data is derived from absolute quantification based on standard curves.
We provide a complete project report containing raw data, QC reports, metabolite concentration tables, and basic statistical analysis results. Personalized in-depth analysis is available upon request.
We offer professional data interpretation and follow-up scientific consulting services, providing support through to publication.
TMAO serves as a crucial molecular bridge connecting diet, the gut microbiome, and systemic host health. As research advances, the demand for its precise measurement and mechanistic understanding continues to grow. Our professional targeted metabolomics service aims to provide researchers and clinical investigators with reliable, efficient technological tools to jointly advance scientific discovery and translational applications in the fields of metabolic health, disease prevention, and treatment.
