Methylation Proteomics Service
Protein methylation is a widely present and functionally significant post-translational modification that primarily occurs on arginine and lysine residues. This modification includes various forms such as mono-methylation, symmetric di-methylation, asymmetric di-methylation, and tri-methylation, providing a sophisticated and complex mechanism for regulating protein function.
In biological processes, arginine methylation plays a key role in regulating RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Meanwhile, lysine methylation not only plays a central role in histone function regulation and epigenetic control of gene transcription but also participates in many other crucial cellular activities.
Recent research indicates that protein methylation, as a dynamic and reversible process, plays a critical role in cell fate determination, metabolic regulation, and disease development by influencing protein activity, localization, stability, and interactions. Abnormalities in this modification are closely associated with various disease states, particularly demonstrating significant regulatory roles in cancer, neurological disorders, and developmental abnormalities.
Our Services
We provide comprehensive and professional methylation proteomics analysis services, including:
- Methylation Site Identification: Systematically identifies methylated proteins and their precise modification sites in samples using high-specificity antibody enrichment and high-resolution mass spectrometry.
- Quantitative Methylation Proteomics Analysis: Employs advanced quantitative strategies to accurately measure methylation levels under different physiological or pathological conditions, revealing dynamic changes in methylation patterns.
- Pan-Cellular Protein Methylation Profiling: Utilizes optimized proteomics technologies to comprehensively analyze all methylated proteins within cells, constructing a complete methylation landscape.
- Functional Studies of Methylation: Integrates bioinformatics analysis to deeply elucidate the biological functions of methylation, including its effects on protein stability, subcellular localization, and molecular interactions.
Service Advantages
Our methylation proteomics service platform offers the following core strengths:
- High Specificity Enrichment: Employs optimized antibody affinity enrichment methods with high affinity and specificity for methylated peptides, ensuring efficient recovery of low-abundance methylated peptides from complex samples.
- Advanced Analytical Platform: Equipped with high-resolution, high-sensitivity mass spectrometry systems, guaranteeing accuracy and reliability in large-scale methylated peptide identification.
- Comprehensive Coverage Capability: Through optimized peptide separation and enrichment strategies, combined with necessary pre-treatment techniques, significantly increases the number and coverage of identified methylated proteins.
- Professional Bioinformatics Analysis: Provides a complete bioinformatics analysis pipeline, including modified protein and site identification, functional classification, pathway annotation, and protein-protein interaction network analysis.
Workflow
Protein extraction and quantification are performed using standardized protocols based on the sample type.
Protein samples are digested using specific proteases, with multi-enzyme strategies employed to increase coverage.
Methylated peptides from the digest are enriched using methylation-specific antibodies via immunoaffinity purification.
Enriched peptides are separated by high-performance liquid chromatography and analyzed by high-resolution mass spectrometry.
Raw MS data is acquired and processed using professional software for database searching and statistical analysis.
A comprehensive bioinformatics analysis is conducted on the identified methylated proteins and sites to uncover their biological significance.
A detailed technical report is provided, including the experimental procedure, MS parameters, identification results, and bioinformatics analysis content.
Sample Submission Guidelines
To ensure accurate experimental results, please refer to the following sample requirements:
| Sample Type | Minimum Amount Required |
| Plant Leaves | Wet weight ≥ 0.8 g |
| Plant Roots, Stems, Xylem, Phloem, etc. | Wet weight ≥ 10 g |
| Cell Samples | Cell count equivalent to wet weight ≥ 8×10⁷ |
| Tissue Samples (Human, Animal, Microbial) | Wet weight ≥ 300 mg |
| Body Fluid Samples | Serum/Plasma volume ≥ 1 ml, CSF ≥ 1 ml, Urine ≥ 50 ml |
All samples should be stored in liquid nitrogen or at -80°C and shipped with an adequate amount of dry ice to avoid repeated freeze-thaw cycles.
Methylation proteomics provides a powerful tool for deciphering the sophisticated regulation of life processes. We are committed to providing you with professional and reliable technical support for your research, helping you dive deeper into the mysteries of protein modifications.
