Phage Display Library Screening for VHH Antibodies
VHH antibodies are single-domain antibodies derived from the heavy-chain variable regions of camelid antibodies, weighing just 15 kDa compared to 150 kDa for conventional antibodies. Their small size gives them superior tissue penetration and makes engineering modifications easier. Unlike traditional antibodies, VHH antibodies are highly stable to heat and pH extremes and can be expressed efficiently in bacteria or yeast without complex disulfide bonding. Using our phage display libraries, Creative Diagnostics can rapidly screen billions of variants to identify high-affinity, high-specificity VHH antibodies, empowering your target validation, drug discovery, and diagnostic development efforts.
Service Offerings
| Service Item | Description |
| High-Throughput Screening | Combine solid- and solution-phase panning to sift through billions of phage clones rapidly |
| Antibody Production | Quick vector construction and optimized expression to yield ≥95% pure VHH antibodies |
| Activity Validation | Conduct binding tests, specificity checks, and functional neutralization or blocking assays |
| Custom Projects | From receiving target proteins or cells to final validation, we offer full end-to-end screening support |
| Data & Reporting | Deliver sequencing results, binding parameters, functional data, and detailed technical reports |
Key Advantages
- Speed & Accuracy
Solid- and solution-phase enrichment reduces screening time to 2–4 weeks with hit rates over 30%. - Expert Platform
Multiple generations of phage libraries and high-throughput infrastructure, backed by a team specializing in VHH antibody R&D. - Quality Assurance
Rigorous QC at every stage—from sequence verification to functional testing—ensures reliable outcomes. - Flexible Customization
Tailor selection conditions (temperature, pH, competitive ligands) to tackle challenging targets. - End-to-End Management
Dedicated project managers keep you informed at every step, ensuring smooth project progress.
Workflow
We work closely with you to understand your target (protein or cell line) and research goals, then design a tailored screening strategy.
- Solid-phase panning: Immobilize your target to enrich phages with strong binders
- Solution-phase panning: Screen in solution to capture weaker or transient interactions
After multiple rounds of enrichment, we sequence positive clones and pinpoint promising VHH antibody candidates.
We subclone selected sequences into expression vectors and produce purified VHH antibodies in bacterial or mammalian hosts.
We perform binding assays, specificity testing, and neutralization or blocking experiments to confirm each VHH antibody's effectiveness.
You receive all positive VHH antibody sequences, candidate samples, and a comprehensive validation report.
Typical Applications
- Target structure and function studies
- Diagnostic reagent and imaging probe development
- Therapeutic antibody candidate discovery
- Process monitoring and biosensor creation
For more information or a customized quote, please contact our technical team. We are committed to delivering professional, efficient phage display screening solutions for your VHH antibody projects.
