Comprehensive Energy Metabolism Analysis Service
Targeted energy metabolomics is a technique centered on specific metabolic pathways, performing absolute quantification of key metabolites within them. The energy metabolism network (primarily including the TCA cycle, glycolysis, oxidative phosphorylation, and the pentose phosphate pathway) serves as the final common pathway for the oxidation and energy production of major nutrients (carbohydrates, lipids, amino acids) and as a crucial hub linking their metabolism. Its homeostasis or dysregulation directly impacts numerous physiological and pathological processes such as cell proliferation, immune response, and stress adaptation. Utilizing highly sensitive and specific mass spectrometry detection, this service systematically maps the dynamic landscape of energy metabolism, aiding in mechanism research, biomarker discovery, and the development of intervention strategies.
Our Services
1. Comprehensive Metabolite Coverage: Utilizing an LC-MS/MS platform with Multiple Reaction Monitoring (MRM) mode for precise quantification of 40 key energy metabolism molecules, covering the following pathways:
- Tricarboxylic Acid (TCA) Cycle: Key intermediates such as citrate, α-ketoglutarate, succinate, malate.
- Glycolytic Pathway (EMP Pathway): Metabolites including glucose, lactate, pyruvate, glyceraldehyde 3-phosphate.
- Pentose Phosphate Pathway: Metabolites like 6-phosphogluconate, ribose 5-phosphate.
- Oxidative Phosphorylation & Related Metabolism: Energy carriers and coenzymes including ATP, ADP, AMP, NADPH, NADP⁺.
2. Personalized Analysis Plans: Customizable sample preparation and detection protocols based on your sample type (cells, tissues, blood, urine, etc.) and specific research questions.
3. Professional Data Analysis: Delivery of a comprehensive bioinformatics report including metabolite concentration quantification, pathway enrichment analysis, and interpretation of metabolic flux trends.
Service Advantages
Our service is built on a stable and reliable LC-MS/MS platform, employing highly specific MRM technology to ensure the accuracy and reproducibility of trace metabolite detection in complex biological samples. We provide an end-to-end solution encompassing sample preparation, instrumental analysis, and data interpretation. Furthermore, our detection panel is specifically focused on the core energy metabolism network, making it directly applicable to cutting-edge research areas such as cellular metabolic reprogramming and disease mechanism studies.
Workflow
Discuss research objectives to finalize the target metabolite list and sample requirements.
Provide detailed guidelines for sample collection and shipment; perform standardized pre-processing upon receipt.
Conduct targeted metabolomics analysis using LC-MS/MS, with simultaneous insertion of quality control samples to ensure data reliability.
Perform quantification and statistical analysis, delivering a complete report with charts, graphs, and interpretive notes.
Assist in interpreting report findings and provide suggestions for further deepening your research.
Application
- Disease Mechanism Research: Investigating energy metabolic remodeling in cancer, neurodegenerative diseases, metabolic disorders (e.g., diabetes, obesity), and other conditions.
- Drug Discovery & Efficacy Evaluation: Assessing the impact of drug candidates on cellular energy metabolism and identifying potential therapeutic targets.
- Exercise Physiology & Nutrition: Revealing systemic energy metabolic network responses to exercise and dietary interventions.
- Plant & Microbial Metabolic Engineering: Analyzing energy and carbon flux distribution during photosynthesis and biosynthesis processes.
The fine-tuned regulation of energy metabolism remains a major focus in life sciences. Creative Diagnostics is committed to supporting your research with precise targeted metabolomics technology, helping you unravel the mysteries of energy flow and accelerate the generation and translation of scientific achievements.
