Multiplex Cytokine Detection and Analysis Service
Cytokines are key signaling molecules that mediate communication between immune cells, precisely regulating the initiation, intensity, and resolution of immune responses. Under physiological conditions, their expression and secretion are tightly controlled. However, during pathological states such as infection, autoimmune diseases, cancer, and excessive inflammatory responses (e.g., cytokine storms), the cytokine network undergoes characteristic dysregulation.
Quantitative detection of specific cytokine profiles is akin to deciphering the internal communication of the immune system. It directly reflects the pattern, phase, and imbalance of the body's immune response. Our cytokine detection service provides you with accurate and efficient quantitative analysis, serving as a powerful tool for in-depth exploration of disease mechanisms, assessment of immune status, and monitoring of therapeutic responses.
Our Services
Utilizing high-sensitivity multiplex detection technology, Creative Diagnostics offers simultaneous and precise quantification of key cytokines in human serum, plasma, or cell culture supernatant. The table below outlines the core detection items along with their primary biological functions and clinical relevance:
| Detection Item | Primary Type / Alias | Core Function & Clinical Significance |
| IL-1 | Pro-inflammatory Cytokine | A key inducer of fever and the acute phase response; closely associated with autoimmune diseases and acute inflammation. |
| IL-2 | Growth Factor | A critical driver for T cell proliferation and activation; reflects cellular immune function status; used in immunotherapy monitoring. |
| IL-2R | Soluble Receptor | A marker of T cell activation; elevated levels are common in autoimmune diseases, transplant rejection, and certain lymphoproliferative disorders. |
| IL-6 | Pleiotropic Cytokine | A central pro-inflammatory factor that induces acute phase protein synthesis; a core indicator for sepsis, chronic inflammation, and cytokine storms. |
| IL-8 | Chemokine | A potent chemoattractant and activator for neutrophils; plays a significant role in infection, acute inflammation, and the tumor microenvironment. |
| IL-10 | Anti-inflammatory Cytokine | A crucial immunosuppressive factor that negatively regulates inflammatory responses; its dysregulation is linked to chronic infections and autoimmune diseases. |
| TNF-α | Pro-inflammatory Cytokine | A master regulator of inflammatory responses, inducing apoptosis; plays a major role in the pathogenesis of conditions like rheumatoid arthritis and inflammatory bowel disease. |
| IFN-α/β | Type I Interferon | Central mediators of antiviral immunity; often abnormally overexpressed in autoimmune diseases like systemic lupus erythematosus. |
| IFN-γ | Type II Interferon | Produced by activated T cells and NK cells; activates macrophages; a key marker for Th1-type cellular immune responses. |
Note: The above detections can be provided as pre-set panels or in flexible combinations based on project requirements.
Service Advantages
Our core strength lies in providing a high-quality, one-stop cytokine solution. Leveraging a professional detection platform, we enable simultaneous, high-sensitivity quantification of multiple cytokines, effectively conserving precious samples and ensuring data consistency. We offer end-to-end professional support from experimental design and sample testing to data analysis. All processes adhere to stringent quality controls, guaranteeing reliable and accurate results. With a standard turnaround time of approximately one week, we efficiently meet your needs in both basic research and translational medicine studies.
Workflow
Communicate your specific needs with our technical team to confirm the cytokine panel, sample type, quantity, and obtain the detailed Sample Collection & Shipping Guide.
Following the guide, collect non-anticoagulated peripheral blood (for serum separation) or prepare already isolated serum samples. Ship the samples to the designated laboratory using adequate ice packs under 2-8°C cold-chain conditions.
Upon receipt, the laboratory immediately logs, labels, and inspects sample condition (e.g., volume, hemolysis, clots). Samples passing QC proceed to the testing queue.
After pre-processing, samples undergo multiplex cytokine detection on specialized platforms. Following acquisition, technicians perform concentration calculation, data normalization, and preliminary analysis according to standard protocols.
Analyzed results undergo secondary review before being compiled into a standardized test report. The report, containing all cytokine concentrations, reference ranges, and necessary annotations, is delivered electronically within the agreed timeframe (typically 1 week after receiving qualified samples).
Notes
To ensure optimal detection results, please note the following key points:
- Sample Requirements: Non-anticoagulated peripheral blood (for serum separation after clotting) is preferred. Please use sterile blood collection tubes and avoid hemolysis. Freshly separated serum is recommended to be shipped at 2-8°C within 24 hours; for short-term storage, it can be kept at 4°C (for 1-3 days).
- Long-term Storage: For batch analysis, it is advised to aliquot serum samples and store them at -80°C, avoiding repeated freeze-thaw cycles.
- Deliverables: You will receive a comprehensive electronic report containing raw concentration data for all detected factors, standard curve references, and brief analytical notes based on the sample type.
