Services

Autophagy Gene Overexpression/Knockdown Lentiviral Services

Inquiry

Overexpression and knockdown of autophagy-related genes are essential for dissecting autophagy regulation, validating target functions, and supporting drug discovery. Our lentiviral platform delivers robust, reproducible gene modulation to advance autophagy research and therapeutic development.

Service Offerings

1. Gene Knockdown Lentivirus

Design and synthesize shRNA constructs targeting up to three autophagy-related genes
Package lentiviral particles with ≥70% knockdown efficiency for each target

2. Gene Overexpression Lentivirus

Clone full-length ORFs of autophagy genes into eukaryotic expression vectors
Package high-titer lentivirus to achieve stable, high-level gene expression

3. Supporting Reagents

Primary and secondary antibodies for detecting overexpressed or knocked-down proteins
Optional ELISA kits or Western blot reagents for quantitative protein analysis

Key Advantages

Our platform combines:

High Efficiency & Reliability: Knockdown efficiency ≥70%, stable and robust overexpression
Multi-Target Capability: Simultaneously modulate three genes in parallel to streamline studies
Expert Team: Specialists in virology, molecular biology, and biosafety oversee every step
Custom Solutions: Optimize shRNA design, vector backbones, and infection conditions for each project
Rigorous Quality Control: Monitor viral titer, gene expression levels, and cell viability to guarantee safety and data integrity

Workflow

STEP 1Consultation

Define target genes, cell models, and research objectives to tailor the strategy.

STEP 2Vector Design & Construction

Synthesize shRNA or ORF inserts, clone into lentiviral backbones, and verify sequence accuracy.

STEP 3Virus Production & Tittering

Produce lentivirus in packaging cells, quantify viral titer, and optimize transduction parameters.

STEP 4Transduction & Validation

Infect target cells and confirm gene knockdown or overexpression by qPCR, Western blot, or flow cytometry.

STEP 5Data Analysis & Reporting

Compile quantitative results, generate a detailed report, and recommend next steps.

STEP 6Ongoing Support

Offer follow-up consultation for protocol optimization or expanded studies

Typical Applications

Functional validation of autophagy regulators
Target verification in drug screening assays
Mechanistic studies of autophagy pathways
Functional assessment in disease models (e.g., neurodegeneration, cancer)
Gene-drug interaction analyses

Considerations

Cell Line Selection: Transduction efficiency varies by cell type; pilot experiments are recommended.
Biosafety Compliance: All viral work follows Biosafety Level 2 guidelines to ensure laboratory safety.
Biological Replicates: Perform at least three independent experiments for statistical rigor.
Validation Methods: Confirm overexpression by Western blot or immunofluorescence; verify knockdown by qPCR and protein analysis.

For detailed protocols or customized solutions, please contact our technical support team.

The service is for research only, not for clinical use.