Recombinant Enzyme Development Service
Recombinant enzymes are indispensable tools in modern life sciences and industrial biotechnology, with broad uses in molecular cloning, genome editing, proteomics, metabolic engineering, and diagnostic assay development. By expressing your target enzyme in the optimal host system, Creative Diagnostics delivers high-yield, high-purity active proteins and offer options for tag addition, truncation, or post-translational modifications, ensuring compatibility with your downstream workflows.
Service Offerings
Leveraging years of expertise in protein expression and purification across four major platforms—insect cells, mammalian cells, Escherichia coli, and yeast—we customize full-length or truncated, untagged or tagged enzyme products. Whether you need microgram-scale samples for basic research or milligram-scale preparations for process development, we flexibly adjust production volumes (μg–mg range) and rigorously follow standardized SOPs and QC protocols to guarantee activity, purity, and stability.
With our service, you will:
- Rapidly obtain highly active recombinant enzymes, saving valuable optimization time
- Choose the best expression system for your required post-translational modifications and enzyme function
- Customize tags and modifications for streamlined purification and detection
- Receive comprehensive analytical reports to ensure reproducibility and reliability
| System | Post-Translational Modifications | Yield | Timeframe | Cost | Highlights |
| Insect Cells | Moderate; supports phosphorylation | Medium–high (mg) | 2–4 weeks | Medium–high | Ideal for eukaryotic proteins, complex folding, partial glycosylation; robust for toxic proteins |
| Mammalian Cells | Full glycosylation, phosphorylation | Medium (μg–mg) | 4–6 weeks | High | Native human-like modifications; closest to clinical-grade quality |
| E. coli | None; no complex modifications | High (mg–g) | 1–2 weeks | Low | Fast, cost-effective; best for non-modified or simply tagged proteins |
| Yeast | Moderate; simple glycosylation | Medium–high (mg) | 2–3 weeks | Medium | Eukaryotic system with partial glycosylation; balance of speed and yield |
Key Advantages
- Flexible expression options across four major systems
- Proven track record with hundreds of enzyme projects
- Adjustable production scale from micrograms to milligrams
- Standardized SOPs and rigorous QC ensure batch-to-batch consistency
- Custom modifications (phosphorylation, tag removal, buffer optimization) to suit your application
Workflow
Primer design based on your specifications
Gene amplification from human cDNA library
Vector construction for optimal expression
System selection: insect cells, mammalian cells, E. coli, or yeast
Condition optimization: temperature, induction, media
Affinity, ion-exchange, and size-exclusion chromatography
Tag removal, phosphorylation, buffer exchange
Concentration & purity: UV280, SDS–PAGE, HPLC
Activity assays: substrate turnover rate, optimal pH/temperature, metal-ion dependence
Stability testing: thermal denaturation, storage conditions
Flexible yield: μg–mg range
Batch consistency under strict quality management
Enzyme samples (lyophilized or in solution)
Sequence and vector details
Activity & purity report
Considerations
- Please provide accurate gene sequences or homologous information for precise design
- Sign an NDA if proprietary or patent-sensitive sequences are involved
- Enzyme activity is sensitive to pH, temperature, and metal ions—please share optimal condition ranges
- Special requests (e.g., tag type, modification method) may affect cost and timeline; final terms will be in the service agreement
Choose our recombinant enzyme development service to secure high-activity, premium-quality enzymes and accelerate your research and manufacturing goals. Contact us for a tailored proposal and quote.
