High-Quality Overexpression Lysates

In molecular biology, immunology, and target validation research, highly specific and sensitive antibody detection relies on reliable positive controls and reference standards. To help you enhance experimental accuracy and reproducibility, we offer a diverse and premium selection of over-expression lysates and related products. Whether you are screening new antibodies or performing routine immunological quantitative analyses, our products provide highly reliable detection standards for your assays.

Detailed Product Description

To meet the research needs for various target proteins, we have built a comprehensive recombinant protein expression library, offering over-expression products for both full-length and partial-length proteins across numerous targets.

We utilize mainstream and well-established expression systems to ensure efficient protein expression and proper folding:

• Mammalian Cell Expression Systems: Utilizing human HEK293 and HEK293T cell lines ensures that complex proteins achieve post-translational modifications (such as glycosylation) and spatial conformations that closely resemble their native states.
• Prokaryotic Expression Systems: Utilizing the E. coli system is ideal for rapid, high-yield basic protein expression requirements.

Additionally, to greatly facilitate your downstream detection and separation workflows, our overexpressed proteins are carefully engineered with common affinity tags, such as GST and HIS tags.

Product Categories

Based on the expression localization and secretion characteristics of the target protein, we provide the following two formats of over-expression products, delivered in a ready-to-use state to streamline your experimental workflow:

1. Over-expression Lysate: For intracellular or membrane-bound proteins, extracted using an optimized lysis formulation to maximize the preservation of the target's epitopes.
2. Over-expression Cell Culture Supernatant: For secreted proteins, collected directly from optimized cell culture media, providing you with protein samples in a near-physiological secreted state.

Please Note: All over-expression lysates and cell culture supernatant products we provide are for Research Use Only (RUO) and are not intended for clinical diagnosis, therapeutic, or any other purposes. Please use them strictly in accordance with the provided product manuals under standard laboratory protocols.