Protein Propionylation Proteomics Service
Protein propionylation is a newly discovered reversible post-translational modification. This process involves the covalent attachment of a propionyl group (from donors like propionyl-CoA) to lysine residues on substrate proteins, catalyzed by acyltransferases. As a newly identified modification type, propionylation plays important regulatory roles in both eukaryotes and prokaryotes. Research shows that this modification is widely involved in global metabolic regulatory networks and metabolic enzyme activity regulation in bacteria, playing key roles in cellular stress responses, epigenetic regulation, and metabolism-related physiological processes.
Propionylation participates in regulating core cellular life activities by influencing various mechanisms including protein stability, protein subcellular localization, metabolic enzyme activity, transcriptional activity of transcription factors, protein-protein interactions, and protein-DNA interactions. With the rapid development of proteomics and mass spectrometry technologies, propionylation modification, as an important member of the histone lysine acylation modification family, is increasingly demonstrating its research value.
Our Services
We provide comprehensive and professional propionylation proteomics analysis services, including:
- Propionylation Site Identification: Systematically identifies propionylated proteins and their precise modification sites using specific antibody enrichment and high-resolution mass spectrometry.
- Quantitative Propionylation Proteomics Analysis: Employs advanced quantitative strategies to accurately measure propionylation levels under different physiological or pathological conditions, revealing dynamic changes in propionylation patterns.
- Pan-Cellular Protein Propionylation Profiling: Utilizes optimized proteomics technologies to comprehensively analyze all propionylated proteins within cells, constructing a complete propionylation landscape.
- Functional Studies of Propionylation: Integrates bioinformatics analysis to deeply elucidate the biological functions of propionylation, including its effects on metabolic pathways, enzyme activity regulation, and cellular stress responses.
Service Advantages
Our propionylation proteomics service platform offers the following core strengths:
- High-Specificity Enrichment: Uses optimized antibody affinity enrichment methods with high affinity and specificity for propionylated peptides, ensuring efficient recovery of low-abundance propionylated peptides from complex samples.
- Advanced Analytical Platform: Equipped with high-resolution, high-sensitivity mass spectrometry systems, guaranteeing accuracy and reliability in large-scale propionylated peptide identification.
- Comprehensive Coverage: Through optimized peptide separation and enrichment strategies, combined with necessary digestion and fractionation methods, significantly increases the number and coverage of identified propionylated proteins.
- Professional Bioinformatics Analysis: Provides a complete bioinformatics analysis pipeline, including modified protein and site identification, functional classification, metabolic pathway annotation, and protein-protein interaction network analysis.
Workflow
Protein extraction and quantification are performed using standardized protocols based on the sample type.
Protein samples are digested using specific proteases, with multi-enzyme strategies employed to increase coverage.
Propionylated peptides from the digest are enriched using propionylation-specific antibodies via immunoaffinity purification.
Enriched peptides are separated by high-performance liquid chromatography and analyzed by high-resolution mass spectrometry.
Raw MS data is acquired and processed using professional software for database searching and statistical analysis.
A comprehensive bioinformatics analysis is conducted on the identified propionylated proteins and sites to uncover their biological significance.
A detailed technical report is provided, including the experimental procedure, MS parameters, identification results, and bioinformatics analysis content.
Sample Submission Guidelines
To ensure accurate experimental results, please refer to the following sample requirements:
| Sample Type | Minimum Amount Required |
| Plant Leaves | Wet weight ≥ 0.8 g |
| Plant Roots, Stems, Xylem, Phloem, etc. | Wet weight ≥ 10 g |
| Cell Samples | Cell count equivalent to wet weight ≥ 8×10⁷ |
| Tissue Samples (Human, Animal, Microbial) | Wet weight ≥ 300 mg |
| Body Fluid Samples | Serum/Plasma volume ≥ 1 ml, CSF ≥ 1 ml, Urine ≥ 50 ml |
All samples must be stored in liquid nitrogen or at -80°C and shipped with sufficient dry ice to avoid repeated freeze-thaw cycles.
Propionylation proteomics provides a powerful tool for deciphering the sophisticated regulatory mechanisms in living organisms. We look forward to supporting your research with professional and reliable technical services, helping you uncover new insights into protein modifications.
