Services

Constrained Peptide Library Construction

Inquiry

Peptide libraries are a fast, flexible tool across proteomics and related fields: drug discovery, receptor ligand screening, protein–protein interaction studies, protein function analysis, nucleic-acid-binding work, enzyme substrate or inhibitor discovery, epitope mapping, messenger-molecule research, and peptide or protein signaling all rely on high-quality peptide sets. Rather than screening one sequence at a time, a defined library lets a project test many related peptides in parallel and read out which positions and residues matter.

We build peptide libraries by chemical synthesis, which gives control over sequence, purity, and modification that biological display cannot. Each library is planned around the question it must answer, then synthesized, quality-checked, and delivered ready to use.

Six Library Designs

Design	What it contains	Typical use
Overlapping peptide library	Overlapping windows that walk along a protein sequence	Locating the region a binder or activity maps to
Alanine-scanning library	Each position replaced singly with alanine	Identifying residues critical to binding or function
Positional-scanning library	One position fixed per set across the length	Mapping which residue at each site contributes most
Truncation library	Successively shortened forms of a peptide	Finding the minimal active core sequence
Random library	Randomized positions across a defined length	Exploring sequence space for activity
Mixed library	A combination of the above in one set	Multi-angle mapping and optimization together

Library Specifications

Minimum set size – at least 24 peptides per library, scalable to the design.
Peptide length – 5 to 20 amino acids, set by the target region.
Modifications – biotin labeling, fluorescent labeling, non-natural amino acids, and disulfide cyclization on request.
Delivery format – lyophilized peptides supplied in a 96-well plate or tube rack, ready for direct use.
Typical turnaround – about 2 to 3 weeks.

Quality and Delivery

Every peptide is synthesized to a consistent, high-purity standard so that results are not confounded by batch differences, and each peptide is delivered lyophilized in its well or tube to avoid cross-contamination. Mass spectrometry and chromatographic checks are provided for the peptides so the composition and purity of the set are documented on arrival. Because the peptides are supplied ready to use, they can go straight into the next experiment.

How the Libraries Are Used

Epitope mapping – pinpointing the residues a binder recognizes.
High-throughput interaction analysis – testing many peptides against a protein at once.
Peptide microarray work – ordered sets suited to arrayed screening.
Substrate and inhibitor searches – peptides that act as antigens, receptor ligands, antimicrobial agents, or enzyme inhibitors.

Why Build a Defined Peptide Library

A defined, chemically synthesized set answers a structural question cleanly: by holding the framework constant and varying only chosen positions, the library reports which residues drive the behavior of interest. That makes it the standard tool for epitope identification and sequence optimization, where the goal is not merely a positive hit but an understanding of why a peptide works.

The service is for research only, not for clinical use.