Capture/Detection Antibody Pair Preparation Service

Quantitative protein measurement — by sandwich ELISA, chemiluminescence, or lateral flow — depends on two antibodies that work together: one to capture the target and one to detect it. If the two antibodies compete for the same site, or bind in a way that blocks each other, the assay fails no matter how good either antibody is on its own. A matched pair is therefore developed and screened as a pair, not as two antibodies chosen separately.

The core requirement is that the capture and detection antibodies bind non-overlapping epitopes on the target, so that both can be bound at the same time. Confirming this is an experimental step: candidate antibodies are tested in pairwise combinations in the actual assay format, and only the combinations that report the target correctly are advanced.

We prepare capture/detection pairs by screening combinations rather than individual antibodies, so the pair delivered has already been shown to function together.

What the Service Covers

• Antibody pair generation – producing a panel of candidate antibodies against the target to be paired.
• Pairwise screening – testing candidates in combination to find capture and detection antibodies that bind non-overlapping epitopes and do not compete.
• Format compatibility – confirming the selected pair works in the intended format, whether sandwich ELISA, chemiluminescence, or lateral flow.
• Delivery of a validated pair – the capture and detection antibodies supplied with the pairing data that support their use together.

The Pairing Principle

A pair works only when both antibodies can be bound to the target at once and neither interferes with the other.

Workflow

• Define the target and format – agree the protein to be measured and the assay format the pair must work in.
• Generate candidates – produce a panel of antibodies against the target, optionally from different epitope regions.
• Screen as pairs – test combinations as capture and detection to identify pairs that sandwich the target.
• Confirm in format – verify the best pairs under the intended detection format.
• Deliver – supply the validated pair with the data supporting the pairing.

Applications

• Immunoassay and kit development – matched pairs as the core reagents of a quantitative assay.
• Sandwich ELISA – pairs that capture and detect a target in a plate-based format.
• Chemiluminescence assays – high-sensitivity detection requiring a well-behaved pair.
• Lateral flow and rapid tests – pairs adapted to a membrane-based, point-of-use format.

Why Work With Us

A pair either works together or it does not, so we screen candidates as combinations and judge them in the format they will be used in. We do not select two antibodies separately and hope they cooperate; instead we test the pairs, confirm that they bind non-overlapping epitopes, and report the pairing data so the reagent set you receive has already been demonstrated to function.