Storage:
Short-term storage at 4℃, some reagents should be kept at -20℃ when not in use for a long time.
Detection Method:
Colorimetric
Specificity:
This ELISA kit has excellent specificity for the detection of 6-Hydroxydopamine.
Shipping Condition:
Ice packs
Assay Procedure Summary:
1. Prepare the reagents, standards, and samples needed for testing; 2. Aliquot 50 µl of diluted standard into the standard wells; 3. Aliquot 50 µl of Standard Diluent buffer into control (zero) well; 4. Add 50 µL/well sample to the microtiter plate; 5. Then add 50 µL of the prepared detection solution A, incubate 1 h at 37°C; 6. After washing the plate 3 times, add 100 µL of the prepared detection solution B and incubate 30 min at 37°C; 7. After washing the plate 5 times, add 90 µL of substrate solution and incubate 10-20 min at 37°C; 8. Terminated by the addition of 50 µL stop solution, the absorbance was read at 450 nm by a microplate reader.
Precautions before Use:
The kits of this ELISA kit are not assembled, please assemble it before use.
Stop Solution:
The stop solution of this kit contains acid. Please pay attention to safe use and avoid direct contact.
Notice:
In order to reduce the influence of various variables on the experimental results during the experiment, you must strictly control the experimental conditions, and the entire experimental operation process should be completed by the same person.
ELISA Kit Components:
Pre-coated 96-Well Microplate
Standard
Diluent A
Diluent B
Wash Buffer
Detection Solution A
Detection Solution B
Standard Diluent Buffer
TMB Substrate
Stop Solution
ELISA Kit Principle:
This kit is based on competitive enzyme-Linked immuno-sorbent assay technology. An antigen is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the the pre-coated 6-OHDA and the 6-OHDA in the sample with the biotin-labelled antibody. The HRP-conjugated reagent is then added, and the plate is incubated at suitable temperature. After TMB substrate is added, only wells that contain sufficient 6-OHDA will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the 6-OHDA amount bound on the plate. The OD is measured by spectrophotometry at a wavelength of 450nm ± 10nm, from which the concentration of 6-OHDA can be calculated.
Standard Form:
Lyophilized
