Principle:
The detection system facilitates the identification of rabbit IgG antibodies bonded to an antigen in tissue sections. The specific antibody is pinpointed by a UNIVERSAL secondary antibody that is conjugated with a horseradish peroxidase (HRP) polymer, designed to specifically recognize both mouse and rabbit immunoglobulins. The polymer-attached complex is subsequently made visible under a light microscope using HRP-compatible chromogens, such as diaminobenzidine (DAB).,The provided HRP polymer conjugated secondary antibody significantly addresses the limitations commonly experienced with traditional immunohistochemistry (IHC) methods, such as poor or inconsistent antigen staining when identifying low-abundance antigens or in situations of suboptimal antibody-antigen binding. By utilizing an HRP polymer conjugate, the sensitivity of detection is dramatically increased, and the process is simplified. Moreover, the HRP polymer-based amplification method reduces the amount of primary antibody needed and shortens the incubation period of the secondary antibodies.
Notes:
Please thoroughly read this instruction manual before starting the experiment.
Please note that any uncareful maneuvers within the IHC assay may impact the final results.
Negative IHC staining results only indicate that the target antigen was not detected. It would be inappropriate to conclude that the antigen of interest is absent in the samples tested.
Be vigilant to avoid skin and eye contact with the reagent. Should any contact occur, immediately rinse the affected area with plenty of water.
The HRP-polymer system can be used in IHC autostainers.
